Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally-relevant concentrations of dibenzanthracene

Timothy Williams, Huifeng Wu, Eduarda Santos, Jon Ball, Ioanna Katsiadaki, Margaret Brown, Paul Baker, Fernando Ortago, John A. Craft

Research output: Contribution to journalArticle

Abstract

A three-spined stickleback (Gasterosteus aculeatus) cDNA array and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolomics approach, together with individual biomarkers, were employed to investigate the responses of male sticklebacks to polycyclic aromatic hydrocarbon exposure. Fish were exposed to 1,2:5,6-dibenzanthracene (DbA) at concentrations between 0.01 and 50 µg per liter dissolved in the ambient water for four days, and hepatic transcript and metabolite profiles were determined in comparison with those of solvent-exposed controls. Induction of gene expression was apparent for cytochrome P450 1A (CYP1A) and CYP2-family monooxygenases and these responses were strongly correlated with DbA exposure concentrations (for CYP1A r > 0.996).

Original languageEnglish
Pages (from-to)6341-6348
Number of pages8
JournalEnvironmental Science and Technology
Volume43
Issue number16
DOIs
Publication statusPublished - 1 Jul 2009

Keywords

  • cDNA array
  • aquaculture and fisheries
  • stickleback

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