Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally-relevant concentrations of dibenzanthracene

Timothy Williams; Huifeng Wu; Eduarda Santos; Jon Ball; Ioanna Katsiadaki; Margaret Brown; Paul Baker; Fernando Ortago; John A. Craft

doi:10.1021/es9008689

Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally-relevant concentrations of dibenzanthracene

Timothy Williams, Huifeng Wu, Eduarda Santos, Jon Ball, Ioanna Katsiadaki, Margaret Brown, Paul Baker, Fernando Ortago, John A. Craft

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

71 Citations (Scopus)

Abstract

A three-spined stickleback (Gasterosteus aculeatus) cDNA array and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolomics approach, together with individual biomarkers, were employed to investigate the responses of male sticklebacks to polycyclic aromatic hydrocarbon exposure. Fish were exposed to 1,2:5,6-dibenzanthracene (DbA) at concentrations between 0.01 and 50 µg per liter dissolved in the ambient water for four days, and hepatic transcript and metabolite profiles were determined in comparison with those of solvent-exposed controls. Induction of gene expression was apparent for cytochrome P450 1A (CYP1A) and CYP2-family monooxygenases and these responses were strongly correlated with DbA exposure concentrations (for CYP1A r > 0.996).

Original language	English
Pages (from-to)	6341-6348
Number of pages	8
Journal	Environmental Science and Technology
Volume	43
Issue number	16
DOIs	https://doi.org/10.1021/es9008689
Publication status	Published - 1 Jul 2009

Keywords

cDNA array
aquaculture and fisheries
stickleback

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10.1021/es9008689

Cite this

Williams, T., Wu, H., Santos, E., Ball, J., Katsiadaki, I., Brown, M., Baker, P., Ortago, F., & Craft, J. A. (2009). Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally-relevant concentrations of dibenzanthracene. Environmental Science and Technology, 43(16), 6341-6348. https://doi.org/10.1021/es9008689

@article{a8786deb909b4a7f9e1b37befbe6d6f3,

title = "Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally-relevant concentrations of dibenzanthracene",

abstract = "A three-spined stickleback (Gasterosteus aculeatus) cDNA array and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolomics approach, together with individual biomarkers, were employed to investigate the responses of male sticklebacks to polycyclic aromatic hydrocarbon exposure. Fish were exposed to 1,2:5,6-dibenzanthracene (DbA) at concentrations between 0.01 and 50 µg per liter dissolved in the ambient water for four days, and hepatic transcript and metabolite profiles were determined in comparison with those of solvent-exposed controls. Induction of gene expression was apparent for cytochrome P450 1A (CYP1A) and CYP2-family monooxygenases and these responses were strongly correlated with DbA exposure concentrations (for CYP1A r > 0.996).",

keywords = "cDNA array, aquaculture and fisheries, stickleback",

author = "Timothy Williams and Huifeng Wu and Eduarda Santos and Jon Ball and Ioanna Katsiadaki and Margaret Brown and Paul Baker and Fernando Ortago and Craft, {John A.}",

note = "Originally published in: Environmental Science and Technology (2009), 43 (16), pp.6341-6348.",

year = "2009",

month = jul,

day = "1",

doi = "10.1021/es9008689",

language = "English",

volume = "43",

pages = "6341--6348",

journal = "Environmental Science and Technology",

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Williams, T, Wu, H, Santos, E, Ball, J, Katsiadaki, I, Brown, M, Baker, P, Ortago, F & Craft, JA 2009, 'Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally-relevant concentrations of dibenzanthracene', Environmental Science and Technology, vol. 43, no. 16, pp. 6341-6348. https://doi.org/10.1021/es9008689

Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally-relevant concentrations of dibenzanthracene. / Williams, Timothy; Wu, Huifeng; Santos, Eduarda et al.
In: Environmental Science and Technology, Vol. 43, No. 16, 01.07.2009, p. 6341-6348.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally-relevant concentrations of dibenzanthracene

AU - Williams, Timothy

AU - Wu, Huifeng

AU - Santos, Eduarda

AU - Ball, Jon

AU - Katsiadaki, Ioanna

AU - Brown, Margaret

AU - Baker, Paul

AU - Ortago, Fernando

AU - Craft, John A.

N1 - Originally published in: Environmental Science and Technology (2009), 43 (16), pp.6341-6348.

PY - 2009/7/1

Y1 - 2009/7/1

N2 - A three-spined stickleback (Gasterosteus aculeatus) cDNA array and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolomics approach, together with individual biomarkers, were employed to investigate the responses of male sticklebacks to polycyclic aromatic hydrocarbon exposure. Fish were exposed to 1,2:5,6-dibenzanthracene (DbA) at concentrations between 0.01 and 50 µg per liter dissolved in the ambient water for four days, and hepatic transcript and metabolite profiles were determined in comparison with those of solvent-exposed controls. Induction of gene expression was apparent for cytochrome P450 1A (CYP1A) and CYP2-family monooxygenases and these responses were strongly correlated with DbA exposure concentrations (for CYP1A r > 0.996).

AB - A three-spined stickleback (Gasterosteus aculeatus) cDNA array and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolomics approach, together with individual biomarkers, were employed to investigate the responses of male sticklebacks to polycyclic aromatic hydrocarbon exposure. Fish were exposed to 1,2:5,6-dibenzanthracene (DbA) at concentrations between 0.01 and 50 µg per liter dissolved in the ambient water for four days, and hepatic transcript and metabolite profiles were determined in comparison with those of solvent-exposed controls. Induction of gene expression was apparent for cytochrome P450 1A (CYP1A) and CYP2-family monooxygenases and these responses were strongly correlated with DbA exposure concentrations (for CYP1A r > 0.996).

KW - cDNA array

KW - aquaculture and fisheries

KW - stickleback

U2 - 10.1021/es9008689

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JO - Environmental Science and Technology

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ER -

Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally-relevant concentrations of dibenzanthracene

Abstract

Keywords

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