Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 76–85 |
| Journal | Food and Chemical Toxicology |
| Volume | 112 |
| Early online date | 21 Dec 2017 |
| DOIs | |
| Publication status | Published - Feb 2018 |
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Keywords
- ARPE-19; cell death; Gypenosides; inflammation; oxidative stress
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Gypenosides protect retinal pigment epithelium cells from oxidative stress. / Alhasani, Reem Hasaballah; Biswas, Lincoln; Tohari, Ali Mohammad; Zhou, Xinzhi; Reilly, James; He, Jian-Feng; Shu, Xinhua.
In: Food and Chemical Toxicology, Vol. 112, 02.2018, p. 76–85.Research output: Contribution to journal › Article
TY - JOUR
T1 - Gypenosides protect retinal pigment epithelium cells from oxidative stress
AU - Alhasani, Reem Hasaballah
AU - Biswas, Lincoln
AU - Tohari, Ali Mohammad
AU - Zhou, Xinzhi
AU - Reilly, James
AU - He, Jian-Feng
AU - Shu, Xinhua
N1 - Acceptance in SAN AAM: 12m embargo
PY - 2018/2
Y1 - 2018/2
N2 - Oxidative stress plays a critical role in the pathogenesis of retinal degeneration. Gypenosides are the major functional components isolated from Gynostemma pentaphyllum. They have been shown to protect against oxidative stress and inflammation and have also demonstrated a protective effect on experimental optic neuritis. In order to determine the protective properties of gypenosides against oxidative stress in human retinal pigment epithelium (RPE) cells, ARPE-19¿cells were treated with H2O2 or H2O2 plus gypenosides for 24¿h. ARPE-19¿cells co-treated with gypenosides had significantly increased cell viability and decreased cell death rate when compared to cells treated with H2O2 alone. The level of GSH, the activities of SOD and catalase, and the expression of NRF2 and antioxidant genes were notably decreased, while there were marked increases in ROS, MDA and pro-inflammatory cytokines in ARPE-19¿cells exposed to H2O2; co-treatment with gypenosides significantly counteract these changes. Our study suggests that gypenosides protect RPE cells from oxidative damage and offer therapeutic potential for the treatment of retinal degeneration.
AB - Oxidative stress plays a critical role in the pathogenesis of retinal degeneration. Gypenosides are the major functional components isolated from Gynostemma pentaphyllum. They have been shown to protect against oxidative stress and inflammation and have also demonstrated a protective effect on experimental optic neuritis. In order to determine the protective properties of gypenosides against oxidative stress in human retinal pigment epithelium (RPE) cells, ARPE-19¿cells were treated with H2O2 or H2O2 plus gypenosides for 24¿h. ARPE-19¿cells co-treated with gypenosides had significantly increased cell viability and decreased cell death rate when compared to cells treated with H2O2 alone. The level of GSH, the activities of SOD and catalase, and the expression of NRF2 and antioxidant genes were notably decreased, while there were marked increases in ROS, MDA and pro-inflammatory cytokines in ARPE-19¿cells exposed to H2O2; co-treatment with gypenosides significantly counteract these changes. Our study suggests that gypenosides protect RPE cells from oxidative damage and offer therapeutic potential for the treatment of retinal degeneration.
KW - ARPE-19; cell death; Gypenosides; inflammation; oxidative stress
U2 - 10.1016/j.fct.2017.12.037
DO - 10.1016/j.fct.2017.12.037
M3 - Article
VL - 112
SP - 76
EP - 85
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
SN - 0278-6915
ER -