Generation of multispecies oral bacteria biofilm models

Jason L Brown, Mark C Butcher, Chandra Lekha Ramalingam Veena, Safa Chogule, William Johnston, Gordon Ramage

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review


It is well-recognized that oral biofilms that occur in health and disease have a polymicrobial composition, though these are poorly reflected in the literature, with many studies focussing on simple mono-species biofilm model systems. The utility of polymicrobial biofilm model systems is that they more accurately reflect the oral cavity and allow researchers to ask relevant questions in basic science studies, pharmaceutical screening, and investigating inflammatory interactions. Here we describe the detailed methodology of how to sequentially construct and maintain polymicrobial biofilm models pertinent to caries, periodontal disease, and denture stomatitis.

Original languageEnglish
Title of host publicationOral Biology: Molecular Techniques and Applications
EditorsGregory J. Seymour, Mary P. Cullinan, Nicholas C. K. Heng, Paul R. Cooper
Number of pages13
ISBN (Electronic)9781071627808
ISBN (Print)9781071627792
Publication statusE-pub ahead of print - 24 Nov 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
ISSN (Print)1064-3745


  • Biofilm model
  • Denture stomatitis
  • Coculture model
  • Gingivitis
  • In vitro
  • Polymicrobial
  • Dental caries
  • Multispecies
  • Periodontitis
  • Oral health

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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