@inbook{7ffbd031cc5447239a31d2fea85e6a25,
title = "Generation of multispecies oral bacteria biofilm models",
abstract = "It is well-recognized that oral biofilms that occur in health and disease have a polymicrobial composition, though these are poorly reflected in the literature, with many studies focussing on simple mono-species biofilm model systems. The utility of polymicrobial biofilm model systems is that they more accurately reflect the oral cavity and allow researchers to ask relevant questions in basic science studies, pharmaceutical screening, and investigating inflammatory interactions. Here we describe the detailed methodology of how to sequentially construct and maintain polymicrobial biofilm models pertinent to caries, periodontal disease, and denture stomatitis.",
keywords = "Biofilm model, Denture stomatitis, Coculture model, Gingivitis, In vitro, Polymicrobial, Dental caries, Multispecies, Periodontitis, Oral health",
author = "Brown, {Jason L} and Butcher, {Mark C} and Veena, {Chandra Lekha Ramalingam} and Safa Chogule and William Johnston and Gordon Ramage",
note = "{\textcopyright} 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
month = nov,
day = "24",
doi = "10.1007/978-1-0716-2780-8_12",
language = "English",
isbn = "9781071627792",
volume = "2588",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer Nature",
pages = "187--199",
editor = "Seymour, {Gregory J.} and Cullinan, {Mary P.} and Heng, {Nicholas C. K.} and Cooper, {Paul R.}",
booktitle = "Oral Biology: Molecular Techniques and Applications",
address = "United States",
}