Gap27 modulates Cx43 function and increases cell migration in eu/hyperglycaemic and eu/hyperinsulinaemic human skin cells

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Abstract

Modulating connexin (Cx) mediated communication (CMC) increases wound-healing. The effects of Gap27, a connexin mimetic peptide, on CMC and cell migration of human dermal fibroblasts and epidermal keratinocytes in euglycaemia/euinsulinaemia or hyperglycaemia/hyperinsulinaemia was examined. Cells were treated with 5.5 mM glucose and 1 nM insulin, or 25 mM glucose and 10 nM insulin. Dye transfer and western blotting measured connexin function and expression; scrape-wound assays assessed cell migration +/- 100 µM Gap27. Hyperglycaemic/hyperinsulinaemic cells showed reduced CMC compared to euglycaemic/euinsulinaemic cells (P<0.05), which Gap27 further reduced (P<0.001). Fibroblast Cx43 protein levels were increased in hyperglycaemia/hyperinsulinaemia. Gap27 increased fibroblast (P<0.01) and keratinocyte (P<0.05) migration. Despite increased Cx43 in hyperglycaemic/hyperinsulinaemic fibroblasts, mimicking raised Cx43 seen at ulcer edges, Gap27 blocked CMC and augmented cell migration in both fibroblasts and keratinocytes. Connexin mimetic peptides can improve cell motility in skin cells exposed to diabetes drivers and have therapeutic potential.
Original languageEnglish
Publication statusPublished - 2011

Fingerprint

Connexin 43
Connexins
Cell Movement
Fibroblasts
Skin
Communication
Keratinocytes
Hyperinsulinism
Hyperglycemia
Cell Migration Assays
Insulin
Glucose
Peptides
Wound Healing
Ulcer
Coloring Agents
Western Blotting
Wounds and Injuries
Proteins

Keywords

  • connexin
  • cell–cell communication
  • migration

Cite this

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title = "Gap27 modulates Cx43 function and increases cell migration in eu/hyperglycaemic and eu/hyperinsulinaemic human skin cells",
abstract = "Modulating connexin (Cx) mediated communication (CMC) increases wound-healing. The effects of Gap27, a connexin mimetic peptide, on CMC and cell migration of human dermal fibroblasts and epidermal keratinocytes in euglycaemia/euinsulinaemia or hyperglycaemia/hyperinsulinaemia was examined. Cells were treated with 5.5 mM glucose and 1 nM insulin, or 25 mM glucose and 10 nM insulin. Dye transfer and western blotting measured connexin function and expression; scrape-wound assays assessed cell migration +/- 100 µM Gap27. Hyperglycaemic/hyperinsulinaemic cells showed reduced CMC compared to euglycaemic/euinsulinaemic cells (P<0.05), which Gap27 further reduced (P<0.001). Fibroblast Cx43 protein levels were increased in hyperglycaemia/hyperinsulinaemia. Gap27 increased fibroblast (P<0.01) and keratinocyte (P<0.05) migration. Despite increased Cx43 in hyperglycaemic/hyperinsulinaemic fibroblasts, mimicking raised Cx43 seen at ulcer edges, Gap27 blocked CMC and augmented cell migration in both fibroblasts and keratinocytes. Connexin mimetic peptides can improve cell motility in skin cells exposed to diabetes drivers and have therapeutic potential.",
keywords = "connexin, cell–cell communication, migration",
author = "Catherine Wright and Patricia Martin",
year = "2011",
language = "English",

}

TY - CONF

T1 - Gap27 modulates Cx43 function and increases cell migration in eu/hyperglycaemic and eu/hyperinsulinaemic human skin cells

AU - Wright, Catherine

AU - Martin, Patricia

PY - 2011

Y1 - 2011

N2 - Modulating connexin (Cx) mediated communication (CMC) increases wound-healing. The effects of Gap27, a connexin mimetic peptide, on CMC and cell migration of human dermal fibroblasts and epidermal keratinocytes in euglycaemia/euinsulinaemia or hyperglycaemia/hyperinsulinaemia was examined. Cells were treated with 5.5 mM glucose and 1 nM insulin, or 25 mM glucose and 10 nM insulin. Dye transfer and western blotting measured connexin function and expression; scrape-wound assays assessed cell migration +/- 100 µM Gap27. Hyperglycaemic/hyperinsulinaemic cells showed reduced CMC compared to euglycaemic/euinsulinaemic cells (P<0.05), which Gap27 further reduced (P<0.001). Fibroblast Cx43 protein levels were increased in hyperglycaemia/hyperinsulinaemia. Gap27 increased fibroblast (P<0.01) and keratinocyte (P<0.05) migration. Despite increased Cx43 in hyperglycaemic/hyperinsulinaemic fibroblasts, mimicking raised Cx43 seen at ulcer edges, Gap27 blocked CMC and augmented cell migration in both fibroblasts and keratinocytes. Connexin mimetic peptides can improve cell motility in skin cells exposed to diabetes drivers and have therapeutic potential.

AB - Modulating connexin (Cx) mediated communication (CMC) increases wound-healing. The effects of Gap27, a connexin mimetic peptide, on CMC and cell migration of human dermal fibroblasts and epidermal keratinocytes in euglycaemia/euinsulinaemia or hyperglycaemia/hyperinsulinaemia was examined. Cells were treated with 5.5 mM glucose and 1 nM insulin, or 25 mM glucose and 10 nM insulin. Dye transfer and western blotting measured connexin function and expression; scrape-wound assays assessed cell migration +/- 100 µM Gap27. Hyperglycaemic/hyperinsulinaemic cells showed reduced CMC compared to euglycaemic/euinsulinaemic cells (P<0.05), which Gap27 further reduced (P<0.001). Fibroblast Cx43 protein levels were increased in hyperglycaemia/hyperinsulinaemia. Gap27 increased fibroblast (P<0.01) and keratinocyte (P<0.05) migration. Despite increased Cx43 in hyperglycaemic/hyperinsulinaemic fibroblasts, mimicking raised Cx43 seen at ulcer edges, Gap27 blocked CMC and augmented cell migration in both fibroblasts and keratinocytes. Connexin mimetic peptides can improve cell motility in skin cells exposed to diabetes drivers and have therapeutic potential.

KW - connexin

KW - cell–cell communication

KW - migration

M3 - Paper

ER -