Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid

Dejan Karadaglic; Andrew David Wood; Michelle McRobbie; Radovan Stojanovic; Charles Simon Herrington

doi:10.1016/j.canep.2009.10.010

Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid

Dejan Karadaglic, Andrew David Wood, Michelle McRobbie, Radovan Stojanovic, Charles Simon Herrington

Research output: Contribution to journal › Article

Abstract

The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically.

Original language	English
Pages (from-to)	463-468
Number of pages	6
Journal	Cancer Epidemiology
Volume	33
Issue number	6
DOIs	https://doi.org/10.1016/j.canep.2009.10.010
Publication status	Published - Dec 2009

Keywords

cervical neoplasia
fluorescence spectrometry
cell culture

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10.1016/j.canep.2009.10.010

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Karadaglic, D., Wood, A. D., McRobbie, M., Stojanovic, R., & Herrington, C. S. (2009). Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid. Cancer Epidemiology, 33(6), 463-468. https://doi.org/10.1016/j.canep.2009.10.010

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abstract = "The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically. ",

keywords = "cervical neoplasia, fluorescence spectrometry, cell culture",

author = "Dejan Karadaglic and Wood, {Andrew David} and Michelle McRobbie and Radovan Stojanovic and Herrington, {Charles Simon}",

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Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid. / Karadaglic, Dejan; Wood, Andrew David; McRobbie, Michelle; Stojanovic, Radovan; Herrington, Charles Simon.

In: Cancer Epidemiology, Vol. 33, No. 6, 12.2009, p. 463-468.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid

AU - Karadaglic, Dejan

AU - Wood, Andrew David

AU - McRobbie, Michelle

AU - Stojanovic, Radovan

AU - Herrington, Charles Simon

PY - 2009/12

Y1 - 2009/12

N2 - The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically.

AB - The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically.

KW - cervical neoplasia

KW - fluorescence spectrometry

KW - cell culture

U2 - 10.1016/j.canep.2009.10.010

DO - 10.1016/j.canep.2009.10.010

M3 - Article

VL - 33

SP - 463

EP - 468

JO - Cancer Epidemiology

JF - Cancer Epidemiology

SN - 1877-7821

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