Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid

Dejan Karadaglic, Andrew David Wood, Michelle McRobbie, Radovan Stojanovic, Charles Simon Herrington

    Research output: Contribution to journal › Article

    Abstract

    The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically.
    Original languageEnglish
    Pages (from-to)463-468
    Number of pages6
    JournalCancer Epidemiology
    Volume33
    Issue number6
    DOIs
    Publication statusPublished - Dec 2009

    Fingerprint

    phenotype
    Surface Properties
    Fluorescence Spectrometry
    acetic acid
    Cervix Uteri
    Uterine Cervical Neoplasms
    Acetic Acid
    Epithelium
    Fluorescence
    Phenotype
    rafts
    fluorescence
    epithelium
    spectroscopy
    Neoplasms
    cancer
    In Vitro Techniques

    Keywords

    • cervical neoplasia
    • fluorescence spectrometry
    • cell culture

    Cite this

    Karadaglic, Dejan ; Wood, Andrew David ; McRobbie, Michelle ; Stojanovic, Radovan ; Herrington, Charles Simon. / Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid. In: Cancer Epidemiology. 2009 ; Vol. 33, No. 6. pp. 463-468.
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    abstract = "The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically.",
    keywords = "cervical neoplasia, fluorescence spectrometry, cell culture",
    author = "Dejan Karadaglic and Wood, {Andrew David} and Michelle McRobbie and Radovan Stojanovic and Herrington, {Charles Simon}",
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    Karadaglic, D, Wood, AD, McRobbie, M, Stojanovic, R & Herrington, CS 2009, 'Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid', Cancer Epidemiology, vol. 33, no. 6, pp. 463-468. https://doi.org/10.1016/j.canep.2009.10.010

    Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid. / Karadaglic, Dejan; Wood, Andrew David; McRobbie, Michelle; Stojanovic, Radovan; Herrington, Charles Simon.

    In: Cancer Epidemiology, Vol. 33, No. 6, 12.2009, p. 463-468.

    Research output: Contribution to journal › Article

    TY - JOUR

    T1 - Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid

    AU - Karadaglic, Dejan

    AU - Wood, Andrew David

    AU - McRobbie, Michelle

    AU - Stojanovic, Radovan

    AU - Herrington, Charles Simon

    PY - 2009/12

    Y1 - 2009/12

    N2 - The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically.

    AB - The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically.

    KW - cervical neoplasia

    KW - fluorescence spectrometry

    KW - cell culture

    U2 - 10.1016/j.canep.2009.10.010

    DO - 10.1016/j.canep.2009.10.010

    M3 - Article

    VL - 33

    SP - 463

    EP - 468

    JO - Cancer Epidemiology

    JF - Cancer Epidemiology

    SN - 1877-7821

    IS - 6

    ER -

    Karadaglic D, Wood AD, McRobbie M, Stojanovic R, Herrington CS. Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid. Cancer Epidemiology. 2009 Dec;33(6):463-468. https://doi.org/10.1016/j.canep.2009.10.010

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