Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid

Dejan Karadaglic, Andrew David Wood, Michelle McRobbie, Radovan Stojanovic, Charles Simon Herrington

    Research output: Contribution to journalArticle

    Abstract

    The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically.
    Original languageEnglish
    Pages (from-to)463-468
    Number of pages6
    JournalCancer Epidemiology
    Volume33
    Issue number6
    DOIs
    Publication statusPublished - Dec 2009

    Fingerprint

    phenotype
    Surface Properties
    Fluorescence Spectrometry
    acetic acid
    Cervix Uteri
    Uterine Cervical Neoplasms
    Acetic Acid
    Epithelium
    Fluorescence
    Phenotype
    rafts
    fluorescence
    epithelium
    spectroscopy
    Neoplasms
    cancer
    In Vitro Techniques

    Keywords

    • cervical neoplasia
    • fluorescence spectrometry
    • cell culture

    Cite this

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    title = "Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid",
    abstract = "The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically.",
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    language = "English",
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    Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid. / Karadaglic, Dejan; Wood, Andrew David; McRobbie, Michelle; Stojanovic, Radovan; Herrington, Charles Simon.

    In: Cancer Epidemiology, Vol. 33, No. 6, 12.2009, p. 463-468.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Karadaglic, Dejan

    AU - Wood, Andrew David

    AU - McRobbie, Michelle

    AU - Stojanovic, Radovan

    AU - Herrington, Charles Simon

    PY - 2009/12

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    KW - cervical neoplasia

    KW - fluorescence spectrometry

    KW - cell culture

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    JF - Cancer Epidemiology

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