First report of the presence of hepatitis E virus in Scottish harvested shellfish purchased at retail level

Zoe O'Hara, Claire Crossan, John Craft, Linda Scobie

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Abstract

Shellfish samples (n = 310) purchased from local supermarkets were analysed for the presence of hepatitis E virus (HEV) by nested RT-PCR and real-time qRT-PCR. Overall, 2.9% of samples tested positive for the presence of HEV. Phylogenetic analysis of HEV sequences revealed all as being genotype 3 HEV. This is the first report of the detection of HEV in commercially sold shellfish in Scotland. These findings may encourage further research that will help address the gaps in the knowledge in respect to foodborne transmission of HEV in Scotland and the rest of the United Kingdom.
Original languageEnglish
Journalfood and environmental virology
DOIs
Publication statusPublished - 13 Feb 2018

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Hepatitis E virus
Shellfish
shellfish
Scotland
supermarkets
United Kingdom
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
reverse transcriptase polymerase chain reaction
Genotype
sampling
Polymerase Chain Reaction
genotype
phylogeny
Research

Keywords

  • hepatitis E
  • Scottish shellfish
  • virology

Cite this

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title = "First report of the presence of hepatitis E virus in Scottish harvested shellfish purchased at retail level",
abstract = "Shellfish samples (n = 310) purchased from local supermarkets were analysed for the presence of hepatitis E virus (HEV) by nested RT-PCR and real-time qRT-PCR. Overall, 2.9{\%} of samples tested positive for the presence of HEV. Phylogenetic analysis of HEV sequences revealed all as being genotype 3 HEV. This is the first report of the detection of HEV in commercially sold shellfish in Scotland. These findings may encourage further research that will help address the gaps in the knowledge in respect to foodborne transmission of HEV in Scotland and the rest of the United Kingdom.",
keywords = "hepatitis E, Scottish shellfish, virology",
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doi = "10.1007/s12560-018-9337-5",
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AU - O'Hara, Zoe

AU - Crossan, Claire

AU - Craft, John

AU - Scobie, Linda

N1 - Acceptance in SAN Springer OA deal

PY - 2018/2/13

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N2 - Shellfish samples (n = 310) purchased from local supermarkets were analysed for the presence of hepatitis E virus (HEV) by nested RT-PCR and real-time qRT-PCR. Overall, 2.9% of samples tested positive for the presence of HEV. Phylogenetic analysis of HEV sequences revealed all as being genotype 3 HEV. This is the first report of the detection of HEV in commercially sold shellfish in Scotland. These findings may encourage further research that will help address the gaps in the knowledge in respect to foodborne transmission of HEV in Scotland and the rest of the United Kingdom.

AB - Shellfish samples (n = 310) purchased from local supermarkets were analysed for the presence of hepatitis E virus (HEV) by nested RT-PCR and real-time qRT-PCR. Overall, 2.9% of samples tested positive for the presence of HEV. Phylogenetic analysis of HEV sequences revealed all as being genotype 3 HEV. This is the first report of the detection of HEV in commercially sold shellfish in Scotland. These findings may encourage further research that will help address the gaps in the knowledge in respect to foodborne transmission of HEV in Scotland and the rest of the United Kingdom.

KW - hepatitis E

KW - Scottish shellfish

KW - virology

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