The purpose of this study was to use transmission electron microscopy (TEM) to investigate further the ultrastructural details of the collagen fibrils linking the anterior limiting lamina (ALL; Bowman’s membrane) of the human cornea to the anterior stromal lamellae. Six disease-free corneas from donors aged 42 to 82 years were fixed (2% glutaraldehyde in 80 mM sodium cacodylate) and processed for TEM within 72 hours postmortem. A series of overlapping images, at 10,204× magnification, of the central corneal ALL–stroma interface were assembled. The features of the terminal ends of fibril bundles at the interface with the anterior stroma were quantitatively assessed.
- vision sciences