Fibre optic spectrophotometry for the in vitro evaluation of ultraviolet radiation (UVR) spectral transmittance of rabbit corneas

J. E. Walsh, Jan P.G. Bergmanson, L. V. Koehler, Michael Doughty, D. P. Flemming, J. H. Harmey

Research output: Contribution to journalArticlepeer-review

Abstract

A fibre optic spectrophotometer front-end system for measuring corneas
to overcome shortcomings associated with existing instruments was tested.
The system allowed prompt measurement postmortem, minimizing beam
pathlength to reduce the effects of scatter and unwanted refraction and
eliminated optical interfaces and cuvette media. Rabbit corneas were excised
immediately postmortem and placed on a detecting fibre optic coupled to
an Ocean Optics spectrophotometer and illuminated by a deuterium–halogen
source. The compact instrument with its small beam size allowed tissue
profiling at test points across the corneal surface and efficient interchange
for comparison of different tissues.

Original languageEnglish
Pages (from-to)375-388
Number of pages14
JournalPhysiological Measurement
Volume29
Issue number3
DOIs
Publication statusPublished - 1 Mar 2008

Keywords

  • spectrophotometry
  • optometry
  • vision sciences

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