Fibre optic spectrophotometry for the in vitro evaluation of ultraviolet radiation (UVR) spectral transmittance of rabbit corneas

J. E. Walsh; Jan P.G. Bergmanson; L. V. Koehler; Michael Doughty; D. P. Flemming; J. H. Harmey

doi:10.1088/0967-3334/29/3/008

Fibre optic spectrophotometry for the in vitro evaluation of ultraviolet radiation (UVR) spectral transmittance of rabbit corneas

J. E. Walsh, Jan P.G. Bergmanson, L. V. Koehler, Michael Doughty, D. P. Flemming, J. H. Harmey

Vision Sciences

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A fibre optic spectrophotometer front-end system for measuring corneas
to overcome shortcomings associated with existing instruments was tested.
The system allowed prompt measurement postmortem, minimizing beam
pathlength to reduce the effects of scatter and unwanted refraction and
eliminated optical interfaces and cuvette media. Rabbit corneas were excised
immediately postmortem and placed on a detecting fibre optic coupled to
an Ocean Optics spectrophotometer and illuminated by a deuterium–halogen
source. The compact instrument with its small beam size allowed tissue
profiling at test points across the corneal surface and efficient interchange
for comparison of different tissues.

Original language	English
Pages (from-to)	375-388
Number of pages	14
Journal	Physiological Measurement
Volume	29
Issue number	3
DOIs	https://doi.org/10.1088/0967-3334/29/3/008
Publication status	Published - 1 Mar 2008

Keywords

spectrophotometry
optometry
vision sciences

Documents and Links

10.1088/0967-3334/29/3/008

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title = "Fibre optic spectrophotometry for the in vitro evaluation of ultraviolet radiation (UVR) spectral transmittance of rabbit corneas",

abstract = "A fibre optic spectrophotometer front-end system for measuring corneasto overcome shortcomings associated with existing instruments was tested.The system allowed prompt measurement postmortem, minimizing beampathlength to reduce the effects of scatter and unwanted refraction andeliminated optical interfaces and cuvette media. Rabbit corneas were excisedimmediately postmortem and placed on a detecting fibre optic coupled toan Ocean Optics spectrophotometer and illuminated by a deuterium–halogensource. The compact instrument with its small beam size allowed tissueprofiling at test points across the corneal surface and efficient interchangefor comparison of different tissues.",

keywords = "spectrophotometry, optometry, vision sciences",

author = "Walsh, {J. E.} and Bergmanson, {Jan P.G.} and Koehler, {L. V.} and Michael Doughty and Flemming, {D. P.} and Harmey, {J. H.}",

note = "<p>Originally published in: Physiological Measurement (2008), 29 (3), pp.375-388.</p>",

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doi = "10.1088/0967-3334/29/3/008",

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T1 - Fibre optic spectrophotometry for the in vitro evaluation of ultraviolet radiation (UVR) spectral transmittance of rabbit corneas

AU - Walsh, J. E.

AU - Bergmanson, Jan P.G.

AU - Koehler, L. V.

AU - Doughty, Michael

AU - Flemming, D. P.

AU - Harmey, J. H.

N1 - <p>Originally published in: Physiological Measurement (2008), 29 (3), pp.375-388.</p>

PY - 2008/3/1

Y1 - 2008/3/1

N2 - A fibre optic spectrophotometer front-end system for measuring corneasto overcome shortcomings associated with existing instruments was tested.The system allowed prompt measurement postmortem, minimizing beampathlength to reduce the effects of scatter and unwanted refraction andeliminated optical interfaces and cuvette media. Rabbit corneas were excisedimmediately postmortem and placed on a detecting fibre optic coupled toan Ocean Optics spectrophotometer and illuminated by a deuterium–halogensource. The compact instrument with its small beam size allowed tissueprofiling at test points across the corneal surface and efficient interchangefor comparison of different tissues.

AB - A fibre optic spectrophotometer front-end system for measuring corneasto overcome shortcomings associated with existing instruments was tested.The system allowed prompt measurement postmortem, minimizing beampathlength to reduce the effects of scatter and unwanted refraction andeliminated optical interfaces and cuvette media. Rabbit corneas were excisedimmediately postmortem and placed on a detecting fibre optic coupled toan Ocean Optics spectrophotometer and illuminated by a deuterium–halogensource. The compact instrument with its small beam size allowed tissueprofiling at test points across the corneal surface and efficient interchangefor comparison of different tissues.

KW - spectrophotometry

KW - optometry

KW - vision sciences

U2 - 10.1088/0967-3334/29/3/008

DO - 10.1088/0967-3334/29/3/008

M3 - Article

SN - 1361-6579

VL - 29

SP - 375

EP - 388

JO - Physiological Measurement

JF - Physiological Measurement

IS - 3

ER -

Fibre optic spectrophotometry for the in vitro evaluation of ultraviolet radiation (UVR) spectral transmittance of rabbit corneas

Abstract

Keywords

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