Expression of membrane-associated proteins by strains of enteroaggregative Escherichia coli

Janice Spencer, Henrik Chart*, Henry R. Smith, Bernard Rowe

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Certain strains of enteroaggregative Escherichia coli express an outer membrane-associated protein, involved with the adhesion of these bacteria to HEp-2 cells. Strains of enteroaggregative E. coli hybridising with DNA probes for aggregative adhesion, diffuse adhesion and aggregative adhesion fimbriae II expressed an outer membrane-associated protein of 18 kDa regulated by magnesium ions. Strains hybridising with the aggregative adhesion probe only expressed a 20-kDa outer membrane-associated protein regulated by calcium and magnesium. The present study describes two populations of enteroaggregative E. coli which appear to adhere to HEp-2 cells by expressing antigenically distinct, negatively charged membrane-associated proteins.

Original languageEnglish
Pages (from-to)325-330
Number of pages6
JournalFEMS Microbiology Letters
Volume161
Issue number2
DOIs
Publication statusPublished - 1 Apr 1998

Keywords

  • Escherichia coli
  • enteroaggregative
  • membrane-associated outer membrane protein
  • DNA probe

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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