Expression of membrane-associated proteins by strains of enteroaggregative Escherichia coli

Janice Spencer; Henrik Chart; Henry R. Smith; Bernard Rowe

doi:10.1016/S0378-1097(98)00090-1

Expression of membrane-associated proteins by strains of enteroaggregative Escherichia coli

Janice Spencer, Henrik Chart^*, Henry R. Smith, Bernard Rowe

^*Corresponding author for this work

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Certain strains of enteroaggregative Escherichia coli express an outer membrane-associated protein, involved with the adhesion of these bacteria to HEp-2 cells. Strains of enteroaggregative E. coli hybridising with DNA probes for aggregative adhesion, diffuse adhesion and aggregative adhesion fimbriae II expressed an outer membrane-associated protein of 18 kDa regulated by magnesium ions. Strains hybridising with the aggregative adhesion probe only expressed a 20-kDa outer membrane-associated protein regulated by calcium and magnesium. The present study describes two populations of enteroaggregative E. coli which appear to adhere to HEp-2 cells by expressing antigenically distinct, negatively charged membrane-associated proteins.

Original language	English
Pages (from-to)	325-330
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	161
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(98)00090-1
Publication status	Published - 1 Apr 1998

Keywords

Escherichia coli
enteroaggregative
membrane-associated outer membrane protein
DNA probe

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/S0378-1097(98)00090-1

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title = "Expression of membrane-associated proteins by strains of enteroaggregative Escherichia coli",

abstract = "Certain strains of enteroaggregative Escherichia coli express an outer membrane-associated protein, involved with the adhesion of these bacteria to HEp-2 cells. Strains of enteroaggregative E. coli hybridising with DNA probes for aggregative adhesion, diffuse adhesion and aggregative adhesion fimbriae II expressed an outer membrane-associated protein of 18 kDa regulated by magnesium ions. Strains hybridising with the aggregative adhesion probe only expressed a 20-kDa outer membrane-associated protein regulated by calcium and magnesium. The present study describes two populations of enteroaggregative E. coli which appear to adhere to HEp-2 cells by expressing antigenically distinct, negatively charged membrane-associated proteins.",

keywords = "Escherichia coli, enteroaggregative, membrane-associated outer membrane protein, DNA probe",

author = "Janice Spencer and Henrik Chart and Smith, {Henry R.} and Bernard Rowe",

year = "1998",

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day = "1",

doi = "10.1016/S0378-1097(98)00090-1",

language = "English",

volume = "161",

pages = "325--330",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press (OUP)",

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TY - JOUR

T1 - Expression of membrane-associated proteins by strains of enteroaggregative Escherichia coli

AU - Spencer, Janice

AU - Chart, Henrik

AU - Smith, Henry R.

AU - Rowe, Bernard

PY - 1998/4/1

Y1 - 1998/4/1

N2 - Certain strains of enteroaggregative Escherichia coli express an outer membrane-associated protein, involved with the adhesion of these bacteria to HEp-2 cells. Strains of enteroaggregative E. coli hybridising with DNA probes for aggregative adhesion, diffuse adhesion and aggregative adhesion fimbriae II expressed an outer membrane-associated protein of 18 kDa regulated by magnesium ions. Strains hybridising with the aggregative adhesion probe only expressed a 20-kDa outer membrane-associated protein regulated by calcium and magnesium. The present study describes two populations of enteroaggregative E. coli which appear to adhere to HEp-2 cells by expressing antigenically distinct, negatively charged membrane-associated proteins.

AB - Certain strains of enteroaggregative Escherichia coli express an outer membrane-associated protein, involved with the adhesion of these bacteria to HEp-2 cells. Strains of enteroaggregative E. coli hybridising with DNA probes for aggregative adhesion, diffuse adhesion and aggregative adhesion fimbriae II expressed an outer membrane-associated protein of 18 kDa regulated by magnesium ions. Strains hybridising with the aggregative adhesion probe only expressed a 20-kDa outer membrane-associated protein regulated by calcium and magnesium. The present study describes two populations of enteroaggregative E. coli which appear to adhere to HEp-2 cells by expressing antigenically distinct, negatively charged membrane-associated proteins.

KW - Escherichia coli

KW - enteroaggregative

KW - membrane-associated outer membrane protein

KW - DNA probe

U2 - 10.1016/S0378-1097(98)00090-1

DO - 10.1016/S0378-1097(98)00090-1

M3 - Article

C2 - 9570123

AN - SCOPUS:0032523146

VL - 161

SP - 325

EP - 330

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -

Expression of membrane-associated proteins by strains of enteroaggregative Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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