Evidence of severe mitochondrial oxidative stress and a protective effect of low oxygen in mouse models of inherited photoreceptor degeneration

Dafni Vlachantoni, Alexa N. Bramall, Michael P. Murphy, Robert W. Taylor, Xinhua Shu, Brian Tulloch, Theo Van Veen, Douglass M. Turnbull

Research output: Contribution to journalArticle

Abstract

The role of oxidative stress within photoreceptors (PRs) in inherited photoreceptor degeneration (IPD) is unclear. We investigated this question using four IPD mouse models (Pde6brd1/rd1, Pde6batrd1/atrd1, Rho2/2 and Prph2rds/rds) and compared the abundance of reduced glutathione (GSH) and the activity of mitochondrial NADH:ubiquinone oxidoreductase (complex I), which is oxidative stress sensitive, as indirect measures of redox status, in the retinas of wild type and IPD mice. All four IPD mutants had significantly reduced retinal complex I activities (14–29% of wild type) and two showed reduced GSH, at a stage prior to the occurrence of significant cell death, whereas mitochondrial citrate synthase, which is oxidative stress insensitive, was unchanged. We orally administered the mitochondrially targeted anti oxidant MitoQ in order to reduce oxidative stress but without any improvement in retinal complex I activity, GSH or rates of PR degeneration.

Original languageEnglish
Pages (from-to)322-335
Number of pages14
JournalHuman Molecular Genetics
Volume20
Issue number2
DOIs
Publication statusPublished - 1 Jan 2011

Keywords

  • mitochondrial oxidative stress
  • inherited photoreceptor degeneration

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