Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers

Gillian Halket; A. Dinsdale; Niall A. Logan

doi:10.1111/j.1472-765X.2009.02765.x

Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers

Gillian Halket, A. Dinsdale, Niall A. Logan

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

One hundred and nine industrial, environmental and clinical isolates were tested using the BCL card. The card contained 46 substrates for measuring carbon source utilization, enzymatic activities, inhibition by 6·5% NaCl and resistance to the antibiotics kanamycin, oleandomycin and polymyxin B. Identifications were made after 14 h incubation, using a database allowing identification of 42 species of the genera Aneurinibacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus and Virgibacillus. The reference identities of all isolates were authenticated by phenotypic methods, with 16S rRNA gene sequencing used to resolve discrepancies.

Original language	English
Pages (from-to)	120-126
Number of pages	7
Journal	Letters in Applied Microbiology
Volume	50
Issue number	1
DOIs	https://doi.org/10.1111/j.1472-765X.2009.02765.x
Publication status	Published - 1 Jan 2010

Keywords

endospores
bacteriology
Bacillus

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10.1111/j.1472-765X.2009.02765.x

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title = "Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers",

abstract = "One hundred and nine industrial, environmental and clinical isolates were tested using the BCL card. The card contained 46 substrates for measuring carbon source utilization, enzymatic activities, inhibition by 6·5% NaCl and resistance to the antibiotics kanamycin, oleandomycin and polymyxin B. Identifications were made after 14 h incubation, using a database allowing identification of 42 species of the genera Aneurinibacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus and Virgibacillus. The reference identities of all isolates were authenticated by phenotypic methods, with 16S rRNA gene sequencing used to resolve discrepancies.",

keywords = "endospores, bacteriology, Bacillus",

author = "Gillian Halket and A. Dinsdale and Logan, {Niall A.}",

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AU - Halket, Gillian

AU - Dinsdale, A.

AU - Logan, Niall A.

N1 - <p>Originally published in: Letters in Applied Microbiology (2010), 50 (1), pp.120-126.</p>

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N2 - One hundred and nine industrial, environmental and clinical isolates were tested using the BCL card. The card contained 46 substrates for measuring carbon source utilization, enzymatic activities, inhibition by 6·5% NaCl and resistance to the antibiotics kanamycin, oleandomycin and polymyxin B. Identifications were made after 14 h incubation, using a database allowing identification of 42 species of the genera Aneurinibacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus and Virgibacillus. The reference identities of all isolates were authenticated by phenotypic methods, with 16S rRNA gene sequencing used to resolve discrepancies.

AB - One hundred and nine industrial, environmental and clinical isolates were tested using the BCL card. The card contained 46 substrates for measuring carbon source utilization, enzymatic activities, inhibition by 6·5% NaCl and resistance to the antibiotics kanamycin, oleandomycin and polymyxin B. Identifications were made after 14 h incubation, using a database allowing identification of 42 species of the genera Aneurinibacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus and Virgibacillus. The reference identities of all isolates were authenticated by phenotypic methods, with 16S rRNA gene sequencing used to resolve discrepancies.

KW - endospores

KW - bacteriology

KW - Bacillus

U2 - 10.1111/j.1472-765X.2009.02765.x

DO - 10.1111/j.1472-765X.2009.02765.x

M3 - Article

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VL - 50

SP - 120

EP - 126

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 1

ER -

Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers

Abstract

Keywords

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