Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers

Gillian Halket, A. Dinsdale, Niall A. Logan

Research output: Contribution to journalArticle

Abstract

One hundred and nine industrial, environmental and clinical isolates were tested using the BCL card. The card contained 46 substrates for measuring carbon source utilization, enzymatic activities, inhibition by 6·5% NaCl and resistance to the antibiotics kanamycin, oleandomycin and polymyxin B. Identifications were made after 14 h incubation, using a database allowing identification of 42 species of the genera Aneurinibacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus and Virgibacillus. The reference identities of all isolates were authenticated by phenotypic methods, with 16S rRNA gene sequencing used to resolve discrepancies.

Original languageEnglish
Pages (from-to)120-126
Number of pages7
JournalLetters in Applied Microbiology
Volume50
Issue number1
DOIs
Publication statusPublished - 1 Jan 2010

Fingerprint

Virgibacillus
Brevibacillus
Geobacillus
Paenibacillus
Polymyxin B
Microbial Drug Resistance
rRNA Genes
Bacillus
Carbon
Databases
oleandomycin B
bekanamycin

Keywords

  • endospores
  • bacteriology
  • Bacillus

Cite this

Halket, Gillian ; Dinsdale, A. ; Logan, Niall A. / Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers. In: Letters in Applied Microbiology. 2010 ; Vol. 50, No. 1. pp. 120-126.
@article{ebec658a692a4bf993810b8231956615,
title = "Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers",
abstract = "One hundred and nine industrial, environmental and clinical isolates were tested using the BCL card. The card contained 46 substrates for measuring carbon source utilization, enzymatic activities, inhibition by 6·5{\%} NaCl and resistance to the antibiotics kanamycin, oleandomycin and polymyxin B. Identifications were made after 14 h incubation, using a database allowing identification of 42 species of the genera Aneurinibacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus and Virgibacillus. The reference identities of all isolates were authenticated by phenotypic methods, with 16S rRNA gene sequencing used to resolve discrepancies.",
keywords = "endospores, bacteriology, Bacillus",
author = "Gillian Halket and A. Dinsdale and Logan, {Niall A.}",
note = "<p>Originally published in: Letters in Applied Microbiology (2010), 50 (1), pp.120-126.</p>",
year = "2010",
month = "1",
day = "1",
doi = "10.1111/j.1472-765X.2009.02765.x",
language = "English",
volume = "50",
pages = "120--126",
number = "1",

}

Halket, G, Dinsdale, A & Logan, NA 2010, 'Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers', Letters in Applied Microbiology, vol. 50, no. 1, pp. 120-126. https://doi.org/10.1111/j.1472-765X.2009.02765.x

Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers. / Halket, Gillian; Dinsdale, A.; Logan, Niall A.

In: Letters in Applied Microbiology, Vol. 50, No. 1, 01.01.2010, p. 120-126.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers

AU - Halket, Gillian

AU - Dinsdale, A.

AU - Logan, Niall A.

N1 - <p>Originally published in: Letters in Applied Microbiology (2010), 50 (1), pp.120-126.</p>

PY - 2010/1/1

Y1 - 2010/1/1

N2 - One hundred and nine industrial, environmental and clinical isolates were tested using the BCL card. The card contained 46 substrates for measuring carbon source utilization, enzymatic activities, inhibition by 6·5% NaCl and resistance to the antibiotics kanamycin, oleandomycin and polymyxin B. Identifications were made after 14 h incubation, using a database allowing identification of 42 species of the genera Aneurinibacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus and Virgibacillus. The reference identities of all isolates were authenticated by phenotypic methods, with 16S rRNA gene sequencing used to resolve discrepancies.

AB - One hundred and nine industrial, environmental and clinical isolates were tested using the BCL card. The card contained 46 substrates for measuring carbon source utilization, enzymatic activities, inhibition by 6·5% NaCl and resistance to the antibiotics kanamycin, oleandomycin and polymyxin B. Identifications were made after 14 h incubation, using a database allowing identification of 42 species of the genera Aneurinibacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus and Virgibacillus. The reference identities of all isolates were authenticated by phenotypic methods, with 16S rRNA gene sequencing used to resolve discrepancies.

KW - endospores

KW - bacteriology

KW - Bacillus

U2 - 10.1111/j.1472-765X.2009.02765.x

DO - 10.1111/j.1472-765X.2009.02765.x

M3 - Article

VL - 50

SP - 120

EP - 126

IS - 1

ER -