The present study evaluated the relationship between estrogenic hormone concentrations (17α-ethinylestradiol and 17β-estradiol) in surface waters in the Metropolitan Area of São Paulo (Brazil) and environmental variables. Four sampling stations were monitored ranging from a protected area to streams discharging human effluent in and around Billings Reservoir. Four sampling campaigns were carried out in each seasonal period: dry and wet. Samples for hormone analysis (in ng L−1) were concentrated (1000×) using solid-phase extraction C18 cartridges and analyzed by liquid chromatography coupled to quadrupole mass spectrometry detection, with 100 ng L−1 limit of quantification. Water temperature, pH, electrical conductivity (EC), and total dissolved solids were determined in situ; total phosphorus and Sinapis alba bioassays were performed subsequently. Reservoir active capacity (AC) and precipitation were also obtained. Estrogenic hormone concentrations were always below limit of quantification at pristine site; at the other sampling stations, 17β-estradiol concentrations varied from below limit of quantification to 1720 ng L−1 and 17α-ethinylestradiol from below limit of quantification to 1200 ng L−1, with the highest concentrations found in the streams discharging into the reservoir. These streams showed higher Pearson’s correlation between 17α-ethinylestradiol, total phosphorus, and electrical conductivity when compared with reservoir stations. Germination index and EC presented negative correlation (Pearson’s r = − 0.61), denoting a phytotoxicity increase with EC increment. AC influenced the dilution of pollutants and showed negative correlations with total phosphorus (Pearson’s r = −0.56). These results highlight the relevance of including streams in water-monitoring programs, since they are important pollutants loads into watersheds.
|Journal||Water, Air, and Soil Pollution|
|Publication status||Published - 23 Mar 2020|
- 17-ethinylestradiol; 17β-estradiol; electrical conductivity; endocrine disruptors; streams monitoring; water pollution
Coelho, L. H. G., de Jesus, T. A., Kohatsu, M. Y., Poccia, G. T., Chicarolli, V., Helwig, K., Hunter, C., Roberts, J., Teedon, P., & Pahl, O. (2020). Estrogenic Hormones in São Paulo Waters (Brazil) and Their Relationship with Environmental Variables and Sinapis alba Phytotoxicity. Water, Air, and Soil Pollution, 231, .