Enhanced sensitivity to hydrogen peroxide induced apoptosis in Evi1 transformed Rat1 fibroblasts due to repression of carbonic anhydrase III

P. Roy, E. Reavey, M. Rayne, S. Roy, M. Abed El Baky, Y. Ishii, Chris Bartholomew

Research output: Contribution to journalArticle

Abstract

EVI1 is a nuclear zinc finger protein essential to normal development, which participates in acute myeloid leukaemia progression and transforms Rat1 fibroblasts. In this study we show that enforced expression of Evi1 in Rat1 fibroblasts protects from paclitaxel-induced apoptosis, consistent with previously published studies. Surprisingly, however, these cells show increased sensitivity to hydrogen peroxide (H2O2)-induced apoptosis, demonstrated by elevated caspase 3 catalytic activity. This effect is caused by a reduction in carbonic anhydrase III (caIII) production.

Original languageEnglish
Pages (from-to)441-452
Number of pages12
JournalFEBS Journal
Volume277
Issue number2
DOIs
Publication statusPublished - 1 Jan 2010

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Keywords

  • Evi1
  • apoptosis
  • carbonic anhydrase III

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