Endothelin mediated contraction of equine laminar veins

John A. Keen; Bruce C. McGorum; Chris Hillier; Jane E. Nally

doi:10.2746/042516408X313634

Endothelin mediated contraction of equine laminar veins

John A. Keen, Bruce C. McGorum, Chris Hillier, Jane E. Nally

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Endothelin-1 (ET-1) may be a key mediator in the pathogenesis of laminitis, but endothelin-mediated responses in the venous microcirculation of the equine foot have yet to be fully characterised. The aim of this study was to characterise the response of equine laminar veins to ET-1 and evaluate the ET-1 receptor subtypes that mediate this response. Small veins (150–500 µm) draining the equine digital laminae from healthy horses and ponies subjected to euthanasia at an abattoir were investigated using wire myography. Concentration response curves were constructed for ET-1 in the presence of ETA (BQ123) and ETB (BQ788) receptor antagonists, and L-NAME, a nitric oxide synthase blocker. The selective ETB receptor agonist BQ3020 was investigated alone and following incubation with L-NAME, with or without BQ788.

Original language	English
Pages (from-to)	488-492
Number of pages	5
Journal	Equine Veterinary Journal
Volume	40
Issue number	5
DOIs	https://doi.org/10.2746/042516408X313634
Publication status	Published - 1 Jul 2008

Keywords

equine biology
laminar veins
vascular
laminitis
endothelin-1

Documents and Links

10.2746/042516408X313634

http://researchonline.gcu.ac.uk/sls/262

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@article{a4edc4b91b9c40b0a3b347ad63350142,

title = "Endothelin mediated contraction of equine laminar veins",

abstract = "Endothelin-1 (ET-1) may be a key mediator in the pathogenesis of laminitis, but endothelin-mediated responses in the venous microcirculation of the equine foot have yet to be fully characterised. The aim of this study was to characterise the response of equine laminar veins to ET-1 and evaluate the ET-1 receptor subtypes that mediate this response. Small veins (150–500 µm) draining the equine digital laminae from healthy horses and ponies subjected to euthanasia at an abattoir were investigated using wire myography. Concentration response curves were constructed for ET-1 in the presence of ETA (BQ123) and ETB (BQ788) receptor antagonists, and L-NAME, a nitric oxide synthase blocker. The selective ETB receptor agonist BQ3020 was investigated alone and following incubation with L-NAME, with or without BQ788.",

keywords = "equine biology, laminar veins, vascular, laminitis, endothelin-1",

author = "Keen, {John A.} and McGorum, {Bruce C.} and Chris Hillier and Nally, {Jane E.}",

note = "Originally published in: Equine Veterinary Journal (2008), 40 (5), pp.488-492.",

year = "2008",

month = jul,

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doi = "10.2746/042516408X313634",

language = "English",

volume = "40",

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journal = "Equine Veterinary Journal",

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TY - JOUR

T1 - Endothelin mediated contraction of equine laminar veins

AU - Keen, John A.

AU - McGorum, Bruce C.

AU - Hillier, Chris

AU - Nally, Jane E.

N1 - Originally published in: Equine Veterinary Journal (2008), 40 (5), pp.488-492.

PY - 2008/7/1

Y1 - 2008/7/1

N2 - Endothelin-1 (ET-1) may be a key mediator in the pathogenesis of laminitis, but endothelin-mediated responses in the venous microcirculation of the equine foot have yet to be fully characterised. The aim of this study was to characterise the response of equine laminar veins to ET-1 and evaluate the ET-1 receptor subtypes that mediate this response. Small veins (150–500 µm) draining the equine digital laminae from healthy horses and ponies subjected to euthanasia at an abattoir were investigated using wire myography. Concentration response curves were constructed for ET-1 in the presence of ETA (BQ123) and ETB (BQ788) receptor antagonists, and L-NAME, a nitric oxide synthase blocker. The selective ETB receptor agonist BQ3020 was investigated alone and following incubation with L-NAME, with or without BQ788.

AB - Endothelin-1 (ET-1) may be a key mediator in the pathogenesis of laminitis, but endothelin-mediated responses in the venous microcirculation of the equine foot have yet to be fully characterised. The aim of this study was to characterise the response of equine laminar veins to ET-1 and evaluate the ET-1 receptor subtypes that mediate this response. Small veins (150–500 µm) draining the equine digital laminae from healthy horses and ponies subjected to euthanasia at an abattoir were investigated using wire myography. Concentration response curves were constructed for ET-1 in the presence of ETA (BQ123) and ETB (BQ788) receptor antagonists, and L-NAME, a nitric oxide synthase blocker. The selective ETB receptor agonist BQ3020 was investigated alone and following incubation with L-NAME, with or without BQ788.

KW - equine biology

KW - laminar veins

KW - vascular

KW - laminitis

KW - endothelin-1

U2 - 10.2746/042516408X313634

DO - 10.2746/042516408X313634

M3 - Article

VL - 40

SP - 488

EP - 492

JO - Equine Veterinary Journal

JF - Equine Veterinary Journal

SN - 0425-1644

IS - 5

ER -