Abstract
Elimination of cholesterol from arterial tissue, crucial in limiting atherogenesis, may be achieved via high-density lipoprotein (HDL)-mediated reverse cholesterol transport (RCT); components of this pathway can be modulated by oxidative stress. Here we have examined the relations between cholesterol efflux, esterification and transfer in human plasma treated with the powerfully reactive nitrogen species, peroxynitrite. Cellular cholesterol efflux to whole plasma, or to peroxynitrite-modified HDL3, was relatively insensitive to peroxynitrite, as was the transfer of esterified cholesterol. However, plasma cholesterol esterification, via lecithin:cholesterol acyltransferase (LCAT), was markedly inhibited, both directly and indirectly, by peroxynitrite treatment, implying inefficient RCT follows HDL sequestration of cellular cholesterol.
Original language | English |
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Pages (from-to) | 327-332 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 431 |
Issue number | 3 |
DOIs | |
Publication status | Published - 24 Jul 1998 |
Externally published | Yes |
Keywords
- Atherogenesis
- Cholesterol efflux
- High-density lipoprotein
- Macrophage
- Peroxynitrite
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology