Effects of peroxynitrite on plasma components of the reverse cholesterol transport pathway

Annette Graham*, Dimitri V. Vinogradov, James S. Owen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Elimination of cholesterol from arterial tissue, crucial in limiting atherogenesis, may be achieved via high-density lipoprotein (HDL)-mediated reverse cholesterol transport (RCT); components of this pathway can be modulated by oxidative stress. Here we have examined the relations between cholesterol efflux, esterification and transfer in human plasma treated with the powerfully reactive nitrogen species, peroxynitrite. Cellular cholesterol efflux to whole plasma, or to peroxynitrite-modified HDL3, was relatively insensitive to peroxynitrite, as was the transfer of esterified cholesterol. However, plasma cholesterol esterification, via lecithin:cholesterol acyltransferase (LCAT), was markedly inhibited, both directly and indirectly, by peroxynitrite treatment, implying inefficient RCT follows HDL sequestration of cellular cholesterol.

Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalFEBS Letters
Volume431
Issue number3
DOIs
Publication statusPublished - 24 Jul 1998
Externally publishedYes

Keywords

  • Atherogenesis
  • Cholesterol efflux
  • High-density lipoprotein
  • Macrophage
  • Peroxynitrite

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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