Abstract
This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17α-ethinyl estradiol (EE2) on erα and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (erα and erβ1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish but EE2 exposure (11ng/L, for 29 days) increased both to a pattern similar to vitellogenic females. The relative expression of three vtg genes (vtga, vtgb and vtgc) along with erα was determined in control and male fish exposed to EE2 (11ng/L) at multiple time-points over 29 days. All four transcripts were significantly induced due to exposure and expression rose during the time course with distinct temporal patterns and vtga reached a substantially higher level at the end of the time course coinciding with rapid elevation in erα expression.
| Original language | English |
|---|---|
| Pages (from-to) | 12-18 |
| Number of pages | 7 |
| Journal | Marine Environmental Research |
| Volume | 96 |
| Early online date | 6 Feb 2014 |
| DOIs | |
| Publication status | Published - May 2014 |
Keywords
- Sand goby
- qPCR
- transcript abundance
- vitellogenin
- endocrine disruption
- 17-ethynyl oestrodiol
- oestrogen receptor
- Pomatoschistus minutus
Documents and Links
- Humble, J et al (2014), Marine Environmental Research article
Originally published in: Marine Environmental Research (2014), 96, pp.12–18. Published by Elsevier, Copyright © 2015 Elsevier B.V. This is a final author version of the article. The final publisher version of the article can be found via the DOI link below.