Effects of 17a-ethinyl estradiol exposure on estrogen receptors a and ß and vitellogenins A, B and C mRNA expression in the liver of sand goby (Pomatoschistus minutus)

Joseph Humble, Minna Saaristo, Kai Lindstrom, Kari Lehtonen, John Craft

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Abstract

This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17a-ethinyl estradiol (EE2) on era and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (era and erß1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish but EE2 exposure (11 ng/L, for 29 days) increased both to a pattern similar to vitellogenic females. The relative expression of three vtg genes (vtga, vtgb and vtgc) along with era was determined in control and male fish exposed to EE2 (11 ng/L) at multiple time-points over 29 days.
Original languageEnglish
Pages (from-to)12-18
Number of pages7
JournalMarine Environmental Research
Volume96
Early online date6 Feb 2014
DOIs
Publication statusPublished - May 2014

Keywords

  • Sand goby
  • qPCR
  • transcript abundance
  • vitellogenin
  • endocrine disruption
  • 17-ethynyl oestrodiol
  • oestrogen receptor
  • Pomatoschistus minutus

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