Effects of 17a-ethinyl estradiol exposure on estrogen receptors a and ß and vitellogenins A, B and C mRNA expression in the liver of sand goby (Pomatoschistus minutus)

Joseph Humble; Minna Saaristo; Kai Lindstrom; Kari Lehtonen; John Craft

doi:10.1016/j.marenvres.2014.01.006

Effects of 17a-ethinyl estradiol exposure on estrogen receptors a and ß and vitellogenins A, B and C mRNA expression in the liver of sand goby (Pomatoschistus minutus)

Joseph Humble, Minna Saaristo, Kai Lindstrom, Kari Lehtonen, John Craft

Biological and Biomedical Sciences

Research output: Contribution to journal › Article

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Abstract

This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17a-ethinyl estradiol (EE2) on era and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (era and erß1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish but EE2 exposure (11 ng/L, for 29 days) increased both to a pattern similar to vitellogenic females. The relative expression of three vtg genes (vtga, vtgb and vtgc) along with era was determined in control and male fish exposed to EE2 (11 ng/L) at multiple time-points over 29 days.

Original language	English
Pages (from-to)	12-18
Number of pages	7
Journal	Marine Environmental Research
Volume	96
Early online date	6 Feb 2014
DOIs	https://doi.org/10.1016/j.marenvres.2014.01.006
Publication status	Published - May 2014

Keywords

Sand goby
qPCR
transcript abundance
vitellogenin
endocrine disruption
17-ethynyl oestrodiol
oestrogen receptor
Pomatoschistus minutus

Documents and Links

10.1016/j.marenvres.2014.01.006

Humble, J et al (2014), Marine Environmental Research article
Originally published in: Marine Environmental Research (2014), 96, pp.12–18. Published by Elsevier, Copyright © 2015 Elsevier B.V. This is a final author version of the article. The final publisher version of the article can be found via the DOI link below.
Accepted author manuscript, 915 KB

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@article{cef50f0720c4499a9a10a2ce9934371b,

title = "Effects of 17a-ethinyl estradiol exposure on estrogen receptors a and {\ss} and vitellogenins A, B and C mRNA expression in the liver of sand goby (Pomatoschistus minutus)",

abstract = "This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17a-ethinyl estradiol (EE2) on era and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (era and er{\ss}1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish but EE2 exposure (11 ng/L, for 29 days) increased both to a pattern similar to vitellogenic females. The relative expression of three vtg genes (vtga, vtgb and vtgc) along with era was determined in control and male fish exposed to EE2 (11 ng/L) at multiple time-points over 29 days. ",

keywords = "Sand goby , qPCR, transcript abundance, vitellogenin, endocrine disruption, 17-ethynyl oestrodiol, oestrogen receptor, Pomatoschistus minutus",

author = "Joseph Humble and Minna Saaristo and Kai Lindstrom and Kari Lehtonen and John Craft",

note = "Library note: This publication was funded by NERC, grant no.NE/G523398/1 Author final draft provided 8-10-15. Project unknown, unable to make Project relation in PURE. Sherpa/Fact - article should be deposited within 12m of publication and should have a 12m embargo. ET 12-10-15",

year = "2014",

month = may,

doi = "10.1016/j.marenvres.2014.01.006",

language = "English",

volume = "96",

pages = "12--18",

journal = "Marine Environmental Research",

issn = "0141-1136",

publisher = "Elsevier B.V.",

}

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T1 - Effects of 17a-ethinyl estradiol exposure on estrogen receptors a and ß and vitellogenins A, B and C mRNA expression in the liver of sand goby (Pomatoschistus minutus)

AU - Humble, Joseph

AU - Saaristo, Minna

AU - Lindstrom, Kai

AU - Lehtonen, Kari

AU - Craft, John

N1 - Library note: This publication was funded by NERC, grant no.NE/G523398/1 Author final draft provided 8-10-15. Project unknown, unable to make Project relation in PURE. Sherpa/Fact - article should be deposited within 12m of publication and should have a 12m embargo. ET 12-10-15

PY - 2014/5

Y1 - 2014/5

N2 - This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17a-ethinyl estradiol (EE2) on era and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (era and erß1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish but EE2 exposure (11 ng/L, for 29 days) increased both to a pattern similar to vitellogenic females. The relative expression of three vtg genes (vtga, vtgb and vtgc) along with era was determined in control and male fish exposed to EE2 (11 ng/L) at multiple time-points over 29 days.

AB - This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17a-ethinyl estradiol (EE2) on era and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (era and erß1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish but EE2 exposure (11 ng/L, for 29 days) increased both to a pattern similar to vitellogenic females. The relative expression of three vtg genes (vtga, vtgb and vtgc) along with era was determined in control and male fish exposed to EE2 (11 ng/L) at multiple time-points over 29 days.

KW - Sand goby

KW - qPCR

KW - transcript abundance

KW - vitellogenin

KW - endocrine disruption

KW - 17-ethynyl oestrodiol

KW - oestrogen receptor

KW - Pomatoschistus minutus

U2 - 10.1016/j.marenvres.2014.01.006

DO - 10.1016/j.marenvres.2014.01.006

M3 - Article

VL - 96

SP - 12

EP - 18

JO - Marine Environmental Research

JF - Marine Environmental Research

SN - 0141-1136

ER -