Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline

Paola  Caruso; Margaret R MacLean; Raya Khanin; John McClure; Elaine Soon; Mark Southgate; Robert A  MacDonald; Jenny A Greig; Keith E Robertson; Rachel Masson; Laura Denby; Yvonne Dempsie; Lu Long; Nicholas W Morrell; Andrew H Baker

doi:10.1161/ATVBAHA.109.202028

Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline

Paola Caruso, Margaret R MacLean, Raya Khanin, John McClure, Elaine Soon, Mark Southgate, Robert A MacDonald, Jenny A Greig, Keith E Robertson, Rachel Masson, Laura Denby, Yvonne Dempsie, Lu Long, Nicholas W Morrell, Andrew H Baker

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

311 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that have the capacity to control protein production through binding "seed" sequences within a target mRNA. Each miRNA is capable of potentially controlling hundreds of genes. The regulation of miRNAs in the lung during the development of pulmonary arterial hypertension (PAH) is unknown. We screened lung miRNA profiles in a longitudinal and crossover design during the development of PAH caused by chronic hypoxia or monocrotaline in rats. We identified reduced expression of Dicer, involved in miRNA processing, during the onset of PAH after hypoxia. MiR-22, miR-30, and let-7f were downregulated, whereas miR-322 and miR-451 were upregulated significantly during the development of PAH in both models.

Original language	English
Pages (from-to)	716-723
Number of pages	8
Journal	Arteriosclerosis, Thrombosis and Vascular Biology
Volume	30
Issue number	4
Early online date	28 Jan 2010
DOIs	https://doi.org/10.1161/ATVBAHA.109.202028
Publication status	Published - 2010

Keywords

pulmonary hypertension
hypoxia
microRNAs

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10.1161/ATVBAHA.109.202028

Cite this

Caruso, P., MacLean, M. R., Khanin, R., McClure, J., Soon, E., Southgate, M., MacDonald, R. A., Greig, J. A., Robertson, K. E., Masson, R., Denby, L., Dempsie, Y., Long, L., Morrell, N. W., & Baker, A. H. (2010). Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline. Arteriosclerosis, Thrombosis and Vascular Biology, 30(4), 716-723. https://doi.org/10.1161/ATVBAHA.109.202028

@article{bc712c409a0245d99bb0d48d0ac4730b,

title = "Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline",

abstract = "MicroRNAs (miRNAs) are small noncoding RNAs that have the capacity to control protein production through binding {"}seed{"} sequences within a target mRNA. Each miRNA is capable of potentially controlling hundreds of genes. The regulation of miRNAs in the lung during the development of pulmonary arterial hypertension (PAH) is unknown. We screened lung miRNA profiles in a longitudinal and crossover design during the development of PAH caused by chronic hypoxia or monocrotaline in rats. We identified reduced expression of Dicer, involved in miRNA processing, during the onset of PAH after hypoxia. MiR-22, miR-30, and let-7f were downregulated, whereas miR-322 and miR-451 were upregulated significantly during the development of PAH in both models. ",

keywords = "pulmonary hypertension , hypoxia, microRNAs",

author = "Paola Caruso and MacLean, {Margaret R} and Raya Khanin and John McClure and Elaine Soon and Mark Southgate and MacDonald, {Robert A} and Greig, {Jenny A} and Robertson, {Keith E} and Rachel Masson and Laura Denby and Yvonne Dempsie and Lu Long and Morrell, {Nicholas W} and Baker, {Andrew H}",

year = "2010",

doi = "10.1161/ATVBAHA.109.202028",

language = "English",

volume = "30",

pages = "716--723",

journal = "Arteriosclerosis, Thrombosis and Vascular Biology",

issn = "1079-5642",

publisher = "Lippincott Williams and Wilkins",

number = "4",

}

Caruso, P, MacLean, MR, Khanin, R, McClure, J, Soon, E, Southgate, M, MacDonald, RA, Greig, JA, Robertson, KE, Masson, R, Denby, L, Dempsie, Y, Long, L, Morrell, NW & Baker, AH 2010, 'Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline', Arteriosclerosis, Thrombosis and Vascular Biology, vol. 30, no. 4, pp. 716-723. https://doi.org/10.1161/ATVBAHA.109.202028

TY - JOUR

T1 - Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline

AU - Caruso, Paola

AU - MacLean, Margaret R

AU - Khanin, Raya

AU - McClure, John

AU - Soon, Elaine

AU - Southgate, Mark

AU - MacDonald, Robert A

AU - Greig, Jenny A

AU - Robertson, Keith E

AU - Masson, Rachel

AU - Denby, Laura

AU - Dempsie, Yvonne

AU - Long, Lu

AU - Morrell, Nicholas W

AU - Baker, Andrew H

PY - 2010

Y1 - 2010

N2 - MicroRNAs (miRNAs) are small noncoding RNAs that have the capacity to control protein production through binding "seed" sequences within a target mRNA. Each miRNA is capable of potentially controlling hundreds of genes. The regulation of miRNAs in the lung during the development of pulmonary arterial hypertension (PAH) is unknown. We screened lung miRNA profiles in a longitudinal and crossover design during the development of PAH caused by chronic hypoxia or monocrotaline in rats. We identified reduced expression of Dicer, involved in miRNA processing, during the onset of PAH after hypoxia. MiR-22, miR-30, and let-7f were downregulated, whereas miR-322 and miR-451 were upregulated significantly during the development of PAH in both models.

AB - MicroRNAs (miRNAs) are small noncoding RNAs that have the capacity to control protein production through binding "seed" sequences within a target mRNA. Each miRNA is capable of potentially controlling hundreds of genes. The regulation of miRNAs in the lung during the development of pulmonary arterial hypertension (PAH) is unknown. We screened lung miRNA profiles in a longitudinal and crossover design during the development of PAH caused by chronic hypoxia or monocrotaline in rats. We identified reduced expression of Dicer, involved in miRNA processing, during the onset of PAH after hypoxia. MiR-22, miR-30, and let-7f were downregulated, whereas miR-322 and miR-451 were upregulated significantly during the development of PAH in both models.

KW - pulmonary hypertension

KW - hypoxia

KW - microRNAs

U2 - 10.1161/ATVBAHA.109.202028

DO - 10.1161/ATVBAHA.109.202028

M3 - Article

SN - 1079-5642

VL - 30

SP - 716

EP - 723

JO - Arteriosclerosis, Thrombosis and Vascular Biology

JF - Arteriosclerosis, Thrombosis and Vascular Biology

IS - 4

ER -

Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline

Abstract

Keywords

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