Dynamic assessment of the floc morphology, bacterial diversity and integron content of an activated sludge reactor processing hospital effluent

Thibault Stalder, Mousaab Alrhmoun, Jean-Noël Louvet, Magali Casellas, Corinne Maftah, Claire Carrion, Marie-Noëlle Pons, Ole Pahl, Marie-Cécile Ploy, Christophe Dagot

Research output: Contribution to journalArticle

Abstract

The treatment of hospital effluents (HE) is a major concern, as they are suspected of disseminating drugs and antibiotic resistance determinants in the environment. In order to assess HE influence on wastewater treatment plant biomass, lab-scale conventional activated sludge systems (CAS) were continuously fed with real HE or urban effluent as a control. To gain insights into the main hurdles linked to HE treatment, we conducted a multiparameter study using classical physicochemical characterization, phase contrast and confocal laser scaning microscopy, and molecular biology (i.e. pyrosequencing) tools. HE caused erosion of floc structure and the production of extracellular polymeric substances attributed to the development of floc-forming bacteria.
Original languageEnglish
Pages (from-to)7909–7917
Number of pages9
JournalEnvironmental Science and Technology
Volume47
Issue number14
Early online date21 Jun 2013
DOIs
Publication statusPublished - 2013

Fingerprint

Sewage sludge
activated sludge
Effluents
effluent
Processing
Effluent treatment
Molecular biology
drug resistance
Antibiotics
antibiotic resistance
Wastewater treatment
Erosion
Bacteria
Microscopic examination
Biomass
hospital
reactor
microscopy
Anti-Bacterial Agents
laser

Keywords

  • hospital effluents
  • antibiotic resistance
  • bacterial diversity

Cite this

Stalder, Thibault ; Alrhmoun, Mousaab ; Louvet, Jean-Noël ; Casellas, Magali ; Maftah, Corinne ; Carrion, Claire ; Pons, Marie-Noëlle ; Pahl, Ole ; Ploy, Marie-Cécile ; Dagot, Christophe . / Dynamic assessment of the floc morphology, bacterial diversity and integron content of an activated sludge reactor processing hospital effluent. In: Environmental Science and Technology. 2013 ; Vol. 47, No. 14. pp. 7909–7917.
@article{f35f806c638946bf8cb0fd7db154acd4,
title = "Dynamic assessment of the floc morphology, bacterial diversity and integron content of an activated sludge reactor processing hospital effluent",
abstract = "The treatment of hospital effluents (HE) is a major concern, as they are suspected of disseminating drugs and antibiotic resistance determinants in the environment. In order to assess HE influence on wastewater treatment plant biomass, lab-scale conventional activated sludge systems (CAS) were continuously fed with real HE or urban effluent as a control. To gain insights into the main hurdles linked to HE treatment, we conducted a multiparameter study using classical physicochemical characterization, phase contrast and confocal laser scaning microscopy, and molecular biology (i.e. pyrosequencing) tools. HE caused erosion of floc structure and the production of extracellular polymeric substances attributed to the development of floc-forming bacteria.",
keywords = "hospital effluents, antibiotic resistance, bacterial diversity",
author = "Thibault Stalder and Mousaab Alrhmoun and Jean-No{\"e}l Louvet and Magali Casellas and Corinne Maftah and Claire Carrion and Marie-No{\"e}lle Pons and Ole Pahl and Marie-C{\'e}cile Ploy and Christophe Dagot",
year = "2013",
doi = "10.1021/es4008646",
language = "English",
volume = "47",
pages = "7909–7917",
journal = "Environmental Science and Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "14",

}

Stalder, T, Alrhmoun, M, Louvet, J-N, Casellas, M, Maftah, C, Carrion, C, Pons, M-N, Pahl, O, Ploy, M-C & Dagot, C 2013, 'Dynamic assessment of the floc morphology, bacterial diversity and integron content of an activated sludge reactor processing hospital effluent', Environmental Science and Technology, vol. 47, no. 14, pp. 7909–7917. https://doi.org/10.1021/es4008646

Dynamic assessment of the floc morphology, bacterial diversity and integron content of an activated sludge reactor processing hospital effluent. / Stalder, Thibault ; Alrhmoun, Mousaab ; Louvet, Jean-Noël ; Casellas, Magali ; Maftah, Corinne ; Carrion, Claire ; Pons, Marie-Noëlle ; Pahl, Ole; Ploy, Marie-Cécile ; Dagot, Christophe .

In: Environmental Science and Technology, Vol. 47, No. 14, 2013, p. 7909–7917.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dynamic assessment of the floc morphology, bacterial diversity and integron content of an activated sludge reactor processing hospital effluent

AU - Stalder, Thibault

AU - Alrhmoun, Mousaab

AU - Louvet, Jean-Noël

AU - Casellas, Magali

AU - Maftah, Corinne

AU - Carrion, Claire

AU - Pons, Marie-Noëlle

AU - Pahl, Ole

AU - Ploy, Marie-Cécile

AU - Dagot, Christophe

PY - 2013

Y1 - 2013

N2 - The treatment of hospital effluents (HE) is a major concern, as they are suspected of disseminating drugs and antibiotic resistance determinants in the environment. In order to assess HE influence on wastewater treatment plant biomass, lab-scale conventional activated sludge systems (CAS) were continuously fed with real HE or urban effluent as a control. To gain insights into the main hurdles linked to HE treatment, we conducted a multiparameter study using classical physicochemical characterization, phase contrast and confocal laser scaning microscopy, and molecular biology (i.e. pyrosequencing) tools. HE caused erosion of floc structure and the production of extracellular polymeric substances attributed to the development of floc-forming bacteria.

AB - The treatment of hospital effluents (HE) is a major concern, as they are suspected of disseminating drugs and antibiotic resistance determinants in the environment. In order to assess HE influence on wastewater treatment plant biomass, lab-scale conventional activated sludge systems (CAS) were continuously fed with real HE or urban effluent as a control. To gain insights into the main hurdles linked to HE treatment, we conducted a multiparameter study using classical physicochemical characterization, phase contrast and confocal laser scaning microscopy, and molecular biology (i.e. pyrosequencing) tools. HE caused erosion of floc structure and the production of extracellular polymeric substances attributed to the development of floc-forming bacteria.

KW - hospital effluents

KW - antibiotic resistance

KW - bacterial diversity

U2 - 10.1021/es4008646

DO - 10.1021/es4008646

M3 - Article

VL - 47

SP - 7909

EP - 7917

JO - Environmental Science and Technology

JF - Environmental Science and Technology

SN - 0013-936X

IS - 14

ER -