Differential expression of central metabotropic glutamate receptor (mGluR) subtypes in a clinical model of post-surgical pain

Sharron Dolan, James G. Kelly, Ana M. Monteiro, Andrea M. Nolan

Research output: Contribution to journalArticle

Abstract

Tissue damage during surgery can induce ‘central sensitization’ and the development of pain and hyperalgesia post-operatively. Metabotropic glutamate receptors (mGluRs) contribute to nociception, inflammatory pain and hyperalgesia. This study characterized the temporal expression of group I (mGluR1, mGluR5) and II (mGluR2, mGluR3) mGluRs in spinal cord following abdominal surgery. Lumbar spinal cord was recovered from adult sheep euthanased 5 h, 1, 2, 3 and 6 days after undergoing a midline laparotomy, and processed for mGluR mRNA (real-time PCR, in situ hybridization) and protein (Western blotting). mGluR5 mRNA was up-regulated 5 h and 1 day post-surgery in laminae I–II of the spinal cord dorsal horn. mGluR5 protein was increased 1 day post-surgery. A delayed induction of mGluR2 and mGluR3 mRNAs and mGluR2/3 protein occurred in spinal cord 3 days after surgery. By 6 days, mGluR2 mRNA levels had returned to normal, however, mGluR3 mRNA and mGluR2/3 protein remained elevated. No change was detected in mGluR1. These results demonstrate that mGluRs are differentially regulated following surgery and support a link between mGluR-mediated activity and post-surgical pain.

Original languageEnglish
JournalPain Journal
DOIs
Publication statusPublished - 1 Jul 2004

Fingerprint

Anatomic Models
Metabotropic Glutamate Receptors
Spinal Cord
Pain
Messenger RNA
Ambulatory Surgical Procedures
Hyperalgesia
Metabotropic Glutamate 5 Receptor
Central Nervous System Sensitization
Proteins
Nociception
Laparotomy
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Sheep
Western Blotting
metabotropic glutamate receptor 2
metabotropic glutamate receptor 3

Keywords

  • differential expression
  • inflammation
  • metabotropic glutamate receptor
  • spinal cord
  • pain

Cite this

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title = "Differential expression of central metabotropic glutamate receptor (mGluR) subtypes in a clinical model of post-surgical pain",
abstract = "Tissue damage during surgery can induce ‘central sensitization’ and the development of pain and hyperalgesia post-operatively. Metabotropic glutamate receptors (mGluRs) contribute to nociception, inflammatory pain and hyperalgesia. This study characterized the temporal expression of group I (mGluR1, mGluR5) and II (mGluR2, mGluR3) mGluRs in spinal cord following abdominal surgery. Lumbar spinal cord was recovered from adult sheep euthanased 5 h, 1, 2, 3 and 6 days after undergoing a midline laparotomy, and processed for mGluR mRNA (real-time PCR, in situ hybridization) and protein (Western blotting). mGluR5 mRNA was up-regulated 5 h and 1 day post-surgery in laminae I–II of the spinal cord dorsal horn. mGluR5 protein was increased 1 day post-surgery. A delayed induction of mGluR2 and mGluR3 mRNAs and mGluR2/3 protein occurred in spinal cord 3 days after surgery. By 6 days, mGluR2 mRNA levels had returned to normal, however, mGluR3 mRNA and mGluR2/3 protein remained elevated. No change was detected in mGluR1. These results demonstrate that mGluRs are differentially regulated following surgery and support a link between mGluR-mediated activity and post-surgical pain.",
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Differential expression of central metabotropic glutamate receptor (mGluR) subtypes in a clinical model of post-surgical pain. / Dolan, Sharron; Kelly, James G.; Monteiro, Ana M.; Nolan, Andrea M.

In: Pain Journal, 01.07.2004.

Research output: Contribution to journalArticle

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