Development of a biomarker for penconazole: a human oral dosing study and a survey of UK residents’ exposure

Craig Sams, Kate Jones, Karen Galea, Laura MacCalman, John Cocker, Paul Teedon, John W. Cherrie, Martie van Tongeren

Research output: Contribution to journalArticle

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Abstract

Penconazole is a widely used fungicide in the UK; however, to date, there have been no peer-reviewed publications reporting human metabolism, excretion or biological monitoring data. The objectives of this study were to i) develop a robust analytical method, ii) determine biomarker levels in volunteers exposed to penconazole, and, finally, to iii) measure the metabolites in samples collected as part of a large investigation of rural residents’ exposure. An LC-MS/MS method was developed for penconazole and two oxidative metabolites. Three volunteers received a single oral dose of 0.03 mg/kg body weight and timed urine samples were collected and analysed. The volunteer study demonstrated that both penconazole-OH and penconazole-COOH are excreted in humans following an oral dose and are viable biomarkers. Excretion is rapid with a half-life of less than four hours. Mean recovery of the administered dose was 47% (range 33%–54%) in urine treated with glucuronidase to hydrolyse any conjugates. The results from the residents’ study showed that levels of penconazole-COOH in this population were low with >80% below the limit of detection. Future sampling strategies that include both end of exposure and next day urine samples, as well as contextual data about the route and time of exposure, are recommended.
Original languageEnglish
Article number10
JournalToxics
Volume4
Issue number2
Early online date13 May 2016
DOIs
Publication statusPublished - May 2016

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Biomarkers
Metabolites
Fungicides
Metabolism
Volunteers
Urine
Sampling
Recovery
Monitoring
Environmental Monitoring
Glucuronidase
Half-Life
Limit of Detection
Publications
penconazole
Surveys and Questionnaires
Body Weight
Population

Keywords

  • penconazole
  • urine
  • biomarkers
  • fungicide
  • spray
  • residents
  • exposure
  • biological monitoring

Cite this

Sams, Craig ; Jones, Kate ; Galea, Karen ; MacCalman, Laura ; Cocker, John ; Teedon, Paul ; Cherrie, John W. ; van Tongeren, Martie. / Development of a biomarker for penconazole: a human oral dosing study and a survey of UK residents’ exposure. In: Toxics. 2016 ; Vol. 4, No. 2.
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abstract = "Penconazole is a widely used fungicide in the UK; however, to date, there have been no peer-reviewed publications reporting human metabolism, excretion or biological monitoring data. The objectives of this study were to i) develop a robust analytical method, ii) determine biomarker levels in volunteers exposed to penconazole, and, finally, to iii) measure the metabolites in samples collected as part of a large investigation of rural residents’ exposure. An LC-MS/MS method was developed for penconazole and two oxidative metabolites. Three volunteers received a single oral dose of 0.03 mg/kg body weight and timed urine samples were collected and analysed. The volunteer study demonstrated that both penconazole-OH and penconazole-COOH are excreted in humans following an oral dose and are viable biomarkers. Excretion is rapid with a half-life of less than four hours. Mean recovery of the administered dose was 47{\%} (range 33{\%}–54{\%}) in urine treated with glucuronidase to hydrolyse any conjugates. The results from the residents’ study showed that levels of penconazole-COOH in this population were low with >80{\%} below the limit of detection. Future sampling strategies that include both end of exposure and next day urine samples, as well as contextual data about the route and time of exposure, are recommended.",
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author = "Craig Sams and Kate Jones and Karen Galea and Laura MacCalman and John Cocker and Paul Teedon and Cherrie, {John W.} and {van Tongeren}, Martie",
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Sams, C, Jones, K, Galea, K, MacCalman, L, Cocker, J, Teedon, P, Cherrie, JW & van Tongeren, M 2016, 'Development of a biomarker for penconazole: a human oral dosing study and a survey of UK residents’ exposure', Toxics, vol. 4, no. 2, 10. https://doi.org/10.3390/toxics4020010

Development of a biomarker for penconazole: a human oral dosing study and a survey of UK residents’ exposure. / Sams, Craig ; Jones, Kate; Galea, Karen; MacCalman, Laura; Cocker, John; Teedon, Paul; Cherrie, John W.; van Tongeren, Martie.

In: Toxics, Vol. 4, No. 2, 10, 05.2016.

Research output: Contribution to journalArticle

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AU - Sams, Craig

AU - Jones, Kate

AU - Galea, Karen

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AU - Cocker, John

AU - Teedon, Paul

AU - Cherrie, John W.

AU - van Tongeren, Martie

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AB - Penconazole is a widely used fungicide in the UK; however, to date, there have been no peer-reviewed publications reporting human metabolism, excretion or biological monitoring data. The objectives of this study were to i) develop a robust analytical method, ii) determine biomarker levels in volunteers exposed to penconazole, and, finally, to iii) measure the metabolites in samples collected as part of a large investigation of rural residents’ exposure. An LC-MS/MS method was developed for penconazole and two oxidative metabolites. Three volunteers received a single oral dose of 0.03 mg/kg body weight and timed urine samples were collected and analysed. The volunteer study demonstrated that both penconazole-OH and penconazole-COOH are excreted in humans following an oral dose and are viable biomarkers. Excretion is rapid with a half-life of less than four hours. Mean recovery of the administered dose was 47% (range 33%–54%) in urine treated with glucuronidase to hydrolyse any conjugates. The results from the residents’ study showed that levels of penconazole-COOH in this population were low with >80% below the limit of detection. Future sampling strategies that include both end of exposure and next day urine samples, as well as contextual data about the route and time of exposure, are recommended.

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KW - urine

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KW - fungicide

KW - spray

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KW - exposure

KW - biological monitoring

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