Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model

Eleanor Townsend, Leighann Sherry, Ranjith Rajendran, Donald Hansom, John Butcher, William MacKay, Craig Williams, Gordon Ramage

Research output: Contribution to journalArticle

19 Downloads (Pure)

Abstract

Chronic diabetic foot ulcers are frequently colonised and infected by polymicrobial biofilms that ultimately prevent healing. This study aimed to create a novel in vitro inter-kingdom wound biofilm model on complex hydrogel-based cellulose substrata to test commonly used topical wound treatments. Inter-kingdom triadic biofilms composed of Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus were shown to be quantitatively greater in this model compared to a simple substratum when assessed by conventional culture, metabolic dye and live dead qPCR. These biofilms were both structurally complex and compositionally dynamic in response to topical therapy, so when treated with either chlorhexidine or povidone iodine, principal component analysis revealed that the 3-D cellulose model was minimally impacted compared to the simple substratum model. This study highlights the importance of biofilm substratum and inclusion of relevant polymicrobial and inter-kingdom components, as these impact penetration and efficacy of topical antiseptics.
Original languageEnglish
Pages (from-to)1259-1270
Number of pages12
JournalBiofouling - The Journal of Bioadhesion and Biofilm Research
Volume32
Issue number10
DOIs
Publication statusPublished - 14 Nov 2016

Fingerprint

Biofilms
biofilm
Wounds and Injuries
Cellulose
cellulose
wound treatment
chlorhexidine
Povidone-Iodine
antiseptics
Diabetic Foot
Local Anti-Infective Agents
Chlorhexidine
Hydrogel
hydrocolloids
iodine
Principal Component Analysis
Candida albicans
Pseudomonas aeruginosa
dyes
Staphylococcus aureus

Keywords

  • chronic wound, in vitro model, polymicrobial, inter kingdom, biofilm

Cite this

Townsend, E., Sherry, L., Rajendran, R., Hansom, D., Butcher, J., MacKay, W., ... Ramage, G. (2016). Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model. Biofouling - The Journal of Bioadhesion and Biofilm Research , 32(10), 1259-1270. https://doi.org/10.1080/08927014.2016.1252337
Townsend, Eleanor ; Sherry, Leighann ; Rajendran, Ranjith ; Hansom, Donald ; Butcher, John ; MacKay, William ; Williams, Craig ; Ramage, Gordon. / Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model. In: Biofouling - The Journal of Bioadhesion and Biofilm Research . 2016 ; Vol. 32, No. 10. pp. 1259-1270.
@article{4011beab293143e6b4e5eb7a7f23353e,
title = "Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model",
abstract = "Chronic diabetic foot ulcers are frequently colonised and infected by polymicrobial biofilms that ultimately prevent healing. This study aimed to create a novel in vitro inter-kingdom wound biofilm model on complex hydrogel-based cellulose substrata to test commonly used topical wound treatments. Inter-kingdom triadic biofilms composed of Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus were shown to be quantitatively greater in this model compared to a simple substratum when assessed by conventional culture, metabolic dye and live dead qPCR. These biofilms were both structurally complex and compositionally dynamic in response to topical therapy, so when treated with either chlorhexidine or povidone iodine, principal component analysis revealed that the 3-D cellulose model was minimally impacted compared to the simple substratum model. This study highlights the importance of biofilm substratum and inclusion of relevant polymicrobial and inter-kingdom components, as these impact penetration and efficacy of topical antiseptics.",
keywords = "chronic wound, in vitro model, polymicrobial, inter kingdom, biofilm",
author = "Eleanor Townsend and Leighann Sherry and Ranjith Rajendran and Donald Hansom and John Butcher and William MacKay and Craig Williams and Gordon Ramage",
note = "AAM: 12m embargo Author not at GCU at time of acceptance/publication Compliant as file deposited in Enlighten - see link to PDF and deposit date",
year = "2016",
month = "11",
day = "14",
doi = "10.1080/08927014.2016.1252337",
language = "English",
volume = "32",
pages = "1259--1270",
number = "10",

}

Townsend, E, Sherry, L, Rajendran, R, Hansom, D, Butcher, J, MacKay, W, Williams, C & Ramage, G 2016, 'Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model', Biofouling - The Journal of Bioadhesion and Biofilm Research , vol. 32, no. 10, pp. 1259-1270. https://doi.org/10.1080/08927014.2016.1252337

Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model. / Townsend, Eleanor; Sherry, Leighann; Rajendran, Ranjith; Hansom, Donald; Butcher, John; MacKay, William; Williams, Craig; Ramage, Gordon.

In: Biofouling - The Journal of Bioadhesion and Biofilm Research , Vol. 32, No. 10, 14.11.2016, p. 1259-1270.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model

AU - Townsend, Eleanor

AU - Sherry, Leighann

AU - Rajendran, Ranjith

AU - Hansom, Donald

AU - Butcher, John

AU - MacKay, William

AU - Williams, Craig

AU - Ramage, Gordon

N1 - AAM: 12m embargo Author not at GCU at time of acceptance/publication Compliant as file deposited in Enlighten - see link to PDF and deposit date

PY - 2016/11/14

Y1 - 2016/11/14

N2 - Chronic diabetic foot ulcers are frequently colonised and infected by polymicrobial biofilms that ultimately prevent healing. This study aimed to create a novel in vitro inter-kingdom wound biofilm model on complex hydrogel-based cellulose substrata to test commonly used topical wound treatments. Inter-kingdom triadic biofilms composed of Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus were shown to be quantitatively greater in this model compared to a simple substratum when assessed by conventional culture, metabolic dye and live dead qPCR. These biofilms were both structurally complex and compositionally dynamic in response to topical therapy, so when treated with either chlorhexidine or povidone iodine, principal component analysis revealed that the 3-D cellulose model was minimally impacted compared to the simple substratum model. This study highlights the importance of biofilm substratum and inclusion of relevant polymicrobial and inter-kingdom components, as these impact penetration and efficacy of topical antiseptics.

AB - Chronic diabetic foot ulcers are frequently colonised and infected by polymicrobial biofilms that ultimately prevent healing. This study aimed to create a novel in vitro inter-kingdom wound biofilm model on complex hydrogel-based cellulose substrata to test commonly used topical wound treatments. Inter-kingdom triadic biofilms composed of Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus were shown to be quantitatively greater in this model compared to a simple substratum when assessed by conventional culture, metabolic dye and live dead qPCR. These biofilms were both structurally complex and compositionally dynamic in response to topical therapy, so when treated with either chlorhexidine or povidone iodine, principal component analysis revealed that the 3-D cellulose model was minimally impacted compared to the simple substratum model. This study highlights the importance of biofilm substratum and inclusion of relevant polymicrobial and inter-kingdom components, as these impact penetration and efficacy of topical antiseptics.

KW - chronic wound, in vitro model, polymicrobial, inter kingdom, biofilm

U2 - 10.1080/08927014.2016.1252337

DO - 10.1080/08927014.2016.1252337

M3 - Article

VL - 32

SP - 1259

EP - 1270

IS - 10

ER -

Townsend E, Sherry L, Rajendran R, Hansom D, Butcher J, MacKay W et al. Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model. Biofouling - The Journal of Bioadhesion and Biofilm Research . 2016 Nov 14;32(10):1259-1270. https://doi.org/10.1080/08927014.2016.1252337