Detection of porcine endogenous retrovirus (PERV) viremia in diseased versus healthy US pigs by qualitative and quantitative Real-Time RT-PCR

N. Pal; R. Baker; S. Schalk; Linda Scobie; A. W. Tucker; T. Opriessnig

doi:10.1111/j.1865-1682.2011.01210.x

Detection of porcine endogenous retrovirus (PERV) viremia in diseased versus healthy US pigs by qualitative and quantitative Real-Time RT-PCR

N. Pal, R. Baker, S. Schalk, Linda Scobie, A. W. Tucker, T. Opriessnig

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Previous studies have linked levels of porcine endogenous retroviruses (PERV)
with poor health and disease in pigs. To determine the levels of expression of
PERVs and their potential association with disease expression, real-time reverse
transcriptase (RT) PCR assays were used to assess PERV-ABC, PERV-C and
PERV-A/C levels in three commercial swine operations in the United States.
Pigs (n = 204) aged 3–25 weeks were screened, and all 369 serum samples
collected were found to be positive for PERV-ABC RNA as expected.

Original language	English
Journal	Transboundary and Emerging Diseases
DOIs	https://doi.org/10.1111/j.1865-1682.2011.01210.x
Publication status	Published - 1 Mar 2011

Keywords

porcine endogenous retroviruses
RT-PCR

Documents and Links

10.1111/j.1865-1682.2011.01210.x

Cite this

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title = "Detection of porcine endogenous retrovirus (PERV) viremia in diseased versus healthy US pigs by qualitative and quantitative Real-Time RT-PCR",

abstract = "Previous studies have linked levels of porcine endogenous retroviruses (PERV)with poor health and disease in pigs. To determine the levels of expression ofPERVs and their potential association with disease expression, real-time reversetranscriptase (RT) PCR assays were used to assess PERV-ABC, PERV-C andPERV-A/C levels in three commercial swine operations in the United States.Pigs (n = 204) aged 3–25 weeks were screened, and all 369 serum samplescollected were found to be positive for PERV-ABC RNA as expected.",

keywords = "porcine endogenous retroviruses, RT-PCR",

author = "N. Pal and R. Baker and S. Schalk and Linda Scobie and Tucker, {A. W.} and T. Opriessnig",

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T1 - Detection of porcine endogenous retrovirus (PERV) viremia in diseased versus healthy US pigs by qualitative and quantitative Real-Time RT-PCR

AU - Pal, N.

AU - Baker, R.

AU - Schalk, S.

AU - Scobie, Linda

AU - Tucker, A. W.

AU - Opriessnig, T.

N1 - <p>Originally published in: Transboundary and Emerging Diseases (2011), available online ahead of print: <a href="http://dx.doi.org/10.1111/j.1865-1682.2011.01210.x">http://dx.doi.org/10.1111/j.1865-1682.2011.01210.x</a></p>

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Previous studies have linked levels of porcine endogenous retroviruses (PERV)with poor health and disease in pigs. To determine the levels of expression ofPERVs and their potential association with disease expression, real-time reversetranscriptase (RT) PCR assays were used to assess PERV-ABC, PERV-C andPERV-A/C levels in three commercial swine operations in the United States.Pigs (n = 204) aged 3–25 weeks were screened, and all 369 serum samplescollected were found to be positive for PERV-ABC RNA as expected.

AB - Previous studies have linked levels of porcine endogenous retroviruses (PERV)with poor health and disease in pigs. To determine the levels of expression ofPERVs and their potential association with disease expression, real-time reversetranscriptase (RT) PCR assays were used to assess PERV-ABC, PERV-C andPERV-A/C levels in three commercial swine operations in the United States.Pigs (n = 204) aged 3–25 weeks were screened, and all 369 serum samplescollected were found to be positive for PERV-ABC RNA as expected.

KW - porcine endogenous retroviruses

KW - RT-PCR

U2 - 10.1111/j.1865-1682.2011.01210.x

DO - 10.1111/j.1865-1682.2011.01210.x

M3 - Article

SN - 1865-1682

JO - Transboundary and Emerging Diseases

JF - Transboundary and Emerging Diseases

ER -

Detection of porcine endogenous retrovirus (PERV) viremia in diseased versus healthy US pigs by qualitative and quantitative Real-Time RT-PCR

Abstract

Keywords

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