Detection of porcine endogenous retrovirus (PERV) viremia in diseased versus healthy US pigs by qualitative and quantitative Real-Time RT-PCR

N. Pal, R. Baker, S. Schalk, Linda Scobie, A. W. Tucker, T. Opriessnig

Research output: Contribution to journalArticle

Abstract

Previous studies have linked levels of porcine endogenous retroviruses (PERV)
with poor health and disease in pigs. To determine the levels of expression of
PERVs and their potential association with disease expression, real-time reverse
transcriptase (RT) PCR assays were used to assess PERV-ABC, PERV-C and
PERV-A/C levels in three commercial swine operations in the United States.
Pigs (n = 204) aged 3–25 weeks were screened, and all 369 serum samples
collected were found to be positive for PERV-ABC RNA as expected.

Original languageEnglish
JournalTransboundary and Emerging Diseases
DOIs
Publication statusPublished - 1 Mar 2011

Fingerprint

Retroviridae
Endogenous Retroviruses
Viremia
viremia
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Swine
swine
swine diseases
RNA
assays

Keywords

  • porcine endogenous retroviruses
  • RT-PCR

Cite this

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title = "Detection of porcine endogenous retrovirus (PERV) viremia in diseased versus healthy US pigs by qualitative and quantitative Real-Time RT-PCR",
abstract = "Previous studies have linked levels of porcine endogenous retroviruses (PERV)with poor health and disease in pigs. To determine the levels of expression ofPERVs and their potential association with disease expression, real-time reversetranscriptase (RT) PCR assays were used to assess PERV-ABC, PERV-C andPERV-A/C levels in three commercial swine operations in the United States.Pigs (n = 204) aged 3–25 weeks were screened, and all 369 serum samplescollected were found to be positive for PERV-ABC RNA as expected.",
keywords = "porcine endogenous retroviruses, RT-PCR",
author = "N. Pal and R. Baker and S. Schalk and Linda Scobie and Tucker, {A. W.} and T. Opriessnig",
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Detection of porcine endogenous retrovirus (PERV) viremia in diseased versus healthy US pigs by qualitative and quantitative Real-Time RT-PCR. / Pal, N.; Baker, R.; Schalk, S.; Scobie, Linda; Tucker, A. W.; Opriessnig, T.

In: Transboundary and Emerging Diseases, 01.03.2011.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of porcine endogenous retrovirus (PERV) viremia in diseased versus healthy US pigs by qualitative and quantitative Real-Time RT-PCR

AU - Pal, N.

AU - Baker, R.

AU - Schalk, S.

AU - Scobie, Linda

AU - Tucker, A. W.

AU - Opriessnig, T.

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AB - Previous studies have linked levels of porcine endogenous retroviruses (PERV)with poor health and disease in pigs. To determine the levels of expression ofPERVs and their potential association with disease expression, real-time reversetranscriptase (RT) PCR assays were used to assess PERV-ABC, PERV-C andPERV-A/C levels in three commercial swine operations in the United States.Pigs (n = 204) aged 3–25 weeks were screened, and all 369 serum samplescollected were found to be positive for PERV-ABC RNA as expected.

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