Detection and enumeration of zoonotic pathogens in agricultural effluents

Mark T. Boyle, Niall A. Logan, Colin Hunter, Ole Pahl

Research output: Contribution to conferencePaper

Abstract

Livestock production is vital to the world economy. However, intensive animal production is known to require large inputs of raw materials and energy, whilst producing greater amounts and concentration of waste material than do the traditional methods of production. Although the wastes, in the form of slurries and manures, are high in plant nutrients and organic matter, they can also contain zoonotic pathogens including Campylobacter, Salmonella and E. coli O157:H7; presenting an important risk of the transmission of infectious diseases into the human population and other animal infection. Comprehensive investigations have been conducted specifically on the important human pathogen Campylobacter, both in its culturable, and its viable but non-culturable forms, using traditional cultivation and contemporary molecular techniques i.e. ImmunoMagnetic Separation (IMS) and Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Results show that 21.6% of surface water samples (n=180) taken at three dairy/beef cattle and sheep farms in Scotland were positive for Campylobacter using the culturing method as detailed below.
Original languageEnglish
Number of pages5
Publication statusPublished - Jun 2013

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Campylobacter
Zoonoses
effluents
Immunomagnetic Separation
Campylobacter coli
Infectious Disease Transmission
Escherichia coli O157
Manure
pathogens
Scotland
Livestock
Salmonella
immunomagnetic separation
Real-Time Polymerase Chain Reaction
Sheep
slurries
livestock production
animal production
Food
beef cattle

Keywords

  • agricultural wastes
  • zoonotic infections
  • cattle
  • Salmonella

Cite this

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title = "Detection and enumeration of zoonotic pathogens in agricultural effluents",
abstract = "Livestock production is vital to the world economy. However, intensive animal production is known to require large inputs of raw materials and energy, whilst producing greater amounts and concentration of waste material than do the traditional methods of production. Although the wastes, in the form of slurries and manures, are high in plant nutrients and organic matter, they can also contain zoonotic pathogens including Campylobacter, Salmonella and E. coli O157:H7; presenting an important risk of the transmission of infectious diseases into the human population and other animal infection. Comprehensive investigations have been conducted specifically on the important human pathogen Campylobacter, both in its culturable, and its viable but non-culturable forms, using traditional cultivation and contemporary molecular techniques i.e. ImmunoMagnetic Separation (IMS) and Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Results show that 21.6{\%} of surface water samples (n=180) taken at three dairy/beef cattle and sheep farms in Scotland were positive for Campylobacter using the culturing method as detailed below.",
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Detection and enumeration of zoonotic pathogens in agricultural effluents. / Boyle, Mark T.; Logan, Niall A.; Hunter, Colin; Pahl, Ole.

2013.

Research output: Contribution to conferencePaper

TY - CONF

T1 - Detection and enumeration of zoonotic pathogens in agricultural effluents

AU - Boyle, Mark T.

AU - Logan, Niall A.

AU - Hunter, Colin

AU - Pahl, Ole

PY - 2013/6

Y1 - 2013/6

N2 - Livestock production is vital to the world economy. However, intensive animal production is known to require large inputs of raw materials and energy, whilst producing greater amounts and concentration of waste material than do the traditional methods of production. Although the wastes, in the form of slurries and manures, are high in plant nutrients and organic matter, they can also contain zoonotic pathogens including Campylobacter, Salmonella and E. coli O157:H7; presenting an important risk of the transmission of infectious diseases into the human population and other animal infection. Comprehensive investigations have been conducted specifically on the important human pathogen Campylobacter, both in its culturable, and its viable but non-culturable forms, using traditional cultivation and contemporary molecular techniques i.e. ImmunoMagnetic Separation (IMS) and Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Results show that 21.6% of surface water samples (n=180) taken at three dairy/beef cattle and sheep farms in Scotland were positive for Campylobacter using the culturing method as detailed below.

AB - Livestock production is vital to the world economy. However, intensive animal production is known to require large inputs of raw materials and energy, whilst producing greater amounts and concentration of waste material than do the traditional methods of production. Although the wastes, in the form of slurries and manures, are high in plant nutrients and organic matter, they can also contain zoonotic pathogens including Campylobacter, Salmonella and E. coli O157:H7; presenting an important risk of the transmission of infectious diseases into the human population and other animal infection. Comprehensive investigations have been conducted specifically on the important human pathogen Campylobacter, both in its culturable, and its viable but non-culturable forms, using traditional cultivation and contemporary molecular techniques i.e. ImmunoMagnetic Separation (IMS) and Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Results show that 21.6% of surface water samples (n=180) taken at three dairy/beef cattle and sheep farms in Scotland were positive for Campylobacter using the culturing method as detailed below.

KW - agricultural wastes

KW - zoonotic infections

KW - cattle

KW - Salmonella

UR - https://www.cabdirect.org/cabdirect/abstract/20163006088

M3 - Paper

ER -