Abstract
Modulating Cx43-mediated communication via gap junctions and/or hemichannels increases skin wound-healing capacity. Cx31.1 expression is associated with apoptosis in eye and ovary. We investigated Cx31.1 and its association with apoptosis in HaCaT scrape-wounded monolayers cultured in normal or ‘diabetic’ levels of glucose and insulin. HaCaTs were treated for 5 days with normal glucose (5.5 mM) and insulin (1 nM; termed NGI) or high glucose (25 mM) and insulin (10 nM; termed HGI). Scrape-wounds (600 µm) were introduced and monitored over 48 hours. Cx31.1 levels were determined by qPCR and immunocytochemistry. Caspase 3/7 activity and cell viability were determined, with some cells pre-exposed to UV. Cx31.1 protein expression was increased in HGI compared to NGI, and was higher in apoptotic than healthy cells in both conditions. Cx31.1 increased in cells at scrape-wound edges over 48 hours. Caspase activity in conditioned media from scrape-wounded cells was higher in HGI than NGI at 0 hours (P < 0.05), but not at later time points. UV exposure significantly increased caspase in both NGI and HGI (P < 0.05), while there was less caspase activity in HGI treated cells pre-UV (P < 0.01). In parallel, cell viability was reduced post-UV exposure in NGI treated cells (P < 0.001), although not in HGI. Cx31.1 expression was modulated in HaCaT scrape-wound closure and was associated with apoptosis, and with ‘diabetic’ levels of glucose and insulin. We propose that Cx31.1 is associated with tissue turn-over in diabetic wounds.
Original language | English |
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Pages | 129 |
Number of pages | 1 |
Publication status | Unpublished - 2013 |
Event | International Gap Junction Conference (IGJC): Connexins, Pannexins and Innexins - Francis Marion Hotel, Charleston, SC, United States Duration: 13 Jul 2013 → 18 Jul 2013 |
Conference
Conference | International Gap Junction Conference (IGJC) |
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Country/Territory | United States |
City | Charleston, SC |
Period | 13/07/13 → 18/07/13 |
Keywords
- apoptosis
- diabetic
- skin