Corneal surface and superficial cells as viewed by scanning electron microscopy and impression cytology sampling

Michael Doughty

doi:10.1097/ICO.0000000000000692

Corneal surface and superficial cells as viewed by scanning electron microscopy and impression cytology sampling

Michael Doughty

Vision Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this research was to objectively compare sizes of corneal epithelial surface cells visualized by scanning electron microscopy (SEM) or collected by impression cytology (IC). Corneas of recent postmortem sheep eyes were either glutaraldehyde fixed for SEM or IC samples taken, glutaraldehyde fixed, and then stained with Giemsa. The corneal epithelial surface was examined at ×200, with the borders of all cells marked and dimensions and areas measured. For SEM, the cells were classified as having light, medium, or dark electron reflex and by the number of cell sides.

Original language	English
Pages (from-to)	243-248
Number of pages	6
Journal	Cornea
Volume	35
Issue number	2
DOIs	https://doi.org/10.1097/ICO.0000000000000692
Publication status	Published - Feb 2016

Keywords

cornea
scanning electron microscopy
impression cytology

Documents and Links

10.1097/ICO.0000000000000692

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abstract = "The aim of this research was to objectively compare sizes of corneal epithelial surface cells visualized by scanning electron microscopy (SEM) or collected by impression cytology (IC). Corneas of recent postmortem sheep eyes were either glutaraldehyde fixed for SEM or IC samples taken, glutaraldehyde fixed, and then stained with Giemsa. The corneal epithelial surface was examined at ×200, with the borders of all cells marked and dimensions and areas measured. For SEM, the cells were classified as having light, medium, or dark electron reflex and by the number of cell sides.",

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