Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysis

Margaret Brown, Timothy Williams, Kevin Chipman, Ioanna Katsiadaki, Matthew Sanders, John A. Craft

Research output: Contribution to journalArticle

Abstract

The three-spined stickleback (Gasterosteus aculeatus) is ideally suited to laboratory studies, while its wide distribution in the northern hemisphere gives it great potential as a sentinel organism. In the setting of a UK-wide collaboration (Fish Toxicogenomics) we have developed a microarray for transcriptomic analysis of chemical responses in populations of G. aculeatus under laboratory and field conditions. Although several EST libraries are available for this species none are from chemical-exposed fish and thus unlikely to include a full set of pollutant-responsive genes.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalMarine Environmental Research
Volume66
Issue number1
DOIs
Publication statusPublished - 1 Jul 2008

Keywords

  • transcriptome analysis
  • cDNA libraries
  • three-spined stickleback

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