Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysis

Margaret Brown; Timothy Williams; Kevin Chipman; Ioanna Katsiadaki; Matthew Sanders; John A. Craft

doi:10.1016/j.marenvres.2008.02.043

Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysis

Margaret Brown, Timothy Williams, Kevin Chipman, Ioanna Katsiadaki, Matthew Sanders, John A. Craft

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The three-spined stickleback (Gasterosteus aculeatus) is ideally suited to laboratory studies, while its wide distribution in the northern hemisphere gives it great potential as a sentinel organism. In the setting of a UK-wide collaboration (Fish Toxicogenomics) we have developed a microarray for transcriptomic analysis of chemical responses in populations of G. aculeatus under laboratory and field conditions. Although several EST libraries are available for this species none are from chemical-exposed fish and thus unlikely to include a full set of pollutant-responsive genes.

Original language	English
Pages (from-to)	127-130
Number of pages	4
Journal	Marine Environmental Research
Volume	66
Issue number	1
DOIs	https://doi.org/10.1016/j.marenvres.2008.02.043
Publication status	Published - 1 Jul 2008

Keywords

transcriptome analysis
cDNA libraries
three-spined stickleback

Documents and Links

10.1016/j.marenvres.2008.02.043

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Brown, M., Williams, T., Chipman, K., Katsiadaki, I., Sanders, M., & Craft, J. A. (2008). Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysis. Marine Environmental Research, 66(1), 127-130. https://doi.org/10.1016/j.marenvres.2008.02.043

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title = "Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysis",

abstract = "The three-spined stickleback (Gasterosteus aculeatus) is ideally suited to laboratory studies, while its wide distribution in the northern hemisphere gives it great potential as a sentinel organism. In the setting of a UK-wide collaboration (Fish Toxicogenomics) we have developed a microarray for transcriptomic analysis of chemical responses in populations of G. aculeatus under laboratory and field conditions. Although several EST libraries are available for this species none are from chemical-exposed fish and thus unlikely to include a full set of pollutant-responsive genes.",

keywords = "transcriptome analysis, cDNA libraries, three-spined stickleback",

author = "Margaret Brown and Timothy Williams and Kevin Chipman and Ioanna Katsiadaki and Matthew Sanders and Craft, {John A.}",

note = "Originally published in: Marine Environmental Research (2008), 66 (1), pp.127-130.",

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doi = "10.1016/j.marenvres.2008.02.043",

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Brown, M, Williams, T, Chipman, K, Katsiadaki, I, Sanders, M & Craft, JA 2008, 'Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysis', Marine Environmental Research, vol. 66, no. 1, pp. 127-130. https://doi.org/10.1016/j.marenvres.2008.02.043

Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysis. / Brown, Margaret; Williams, Timothy; Chipman, Kevin et al.

In: Marine Environmental Research, Vol. 66, No. 1, 01.07.2008, p. 127-130.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysis

AU - Brown, Margaret

AU - Williams, Timothy

AU - Chipman, Kevin

AU - Katsiadaki, Ioanna

AU - Sanders, Matthew

AU - Craft, John A.

N1 - Originally published in: Marine Environmental Research (2008), 66 (1), pp.127-130.

PY - 2008/7/1

Y1 - 2008/7/1

N2 - The three-spined stickleback (Gasterosteus aculeatus) is ideally suited to laboratory studies, while its wide distribution in the northern hemisphere gives it great potential as a sentinel organism. In the setting of a UK-wide collaboration (Fish Toxicogenomics) we have developed a microarray for transcriptomic analysis of chemical responses in populations of G. aculeatus under laboratory and field conditions. Although several EST libraries are available for this species none are from chemical-exposed fish and thus unlikely to include a full set of pollutant-responsive genes.

AB - The three-spined stickleback (Gasterosteus aculeatus) is ideally suited to laboratory studies, while its wide distribution in the northern hemisphere gives it great potential as a sentinel organism. In the setting of a UK-wide collaboration (Fish Toxicogenomics) we have developed a microarray for transcriptomic analysis of chemical responses in populations of G. aculeatus under laboratory and field conditions. Although several EST libraries are available for this species none are from chemical-exposed fish and thus unlikely to include a full set of pollutant-responsive genes.

KW - transcriptome analysis

KW - cDNA libraries

KW - three-spined stickleback

U2 - 10.1016/j.marenvres.2008.02.043

DO - 10.1016/j.marenvres.2008.02.043

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VL - 66

SP - 127

EP - 130

JO - Marine Environmental Research

JF - Marine Environmental Research

SN - 0141-1136

IS - 1

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Brown M, Williams T, Chipman K, Katsiadaki I, Sanders M, Craft JA. Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysis. Marine Environmental Research. 2008 Jul 1;66(1):127-130. https://doi.org/10.1016/j.marenvres.2008.02.043