Comparison or- fl.uorophores in fll'orophotometric analysis of human thar turnover

J. P. Craig*, A. Tomlinson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Fluorophotomemc analvsis ol' hunuin tear turnover rates (TTRs) can he performed llith fluorescent agents ol differing composition and concentration. This study compared tear turnover rates derived frnm measurements made with the FM-2 FluorotronIM Ma.stei using conventional (sodium fluorescein (lc/> and 2'7c)) and high molecular weighl fluoiescem iFluorosoftIM (0 35(/ )).
Ten normal subjects i5 males and 5 females: mean age of 35.2 ±8.5 years] participated in the studl. A l|al drop of each of the thiee lluorescent agents was instilled into the left eye on three consecutive days- m a double-masked, randomised manner Subjects reported am discomfort on instillation ol (he agent. TTRs were calculated from the fluorophotometiic data with commercially-available software. Data from the first 5 minutes post-instillaiion were considered as mdicaiiiit; the reflex tear flow, and those from 5 to 20 minutes, basai teai floll. Results
Basal TTRs uere found to he l'.4± 5')'. tU.7±A.Cf and 9.8 ±7.0r/r with the 1# and 2C''t sodium fluoresccm and the l-ïnoresolïIM. respectively. Repeated measures ANOVA showed intcisub|eel lariution in basal TTR as significant (p< 0.0011 but no difference was observed within subjects for the three lluorophores (p - 0.595). Reflex TTR values were U.2±1297(, 7.0 ±20.6 c/< and MA±< for the lConclusions
Basal TTR was unaffected by the different lluorophores used in the current study Fïïïoresofi™ was found to nntate the ociilai surface in some cases and increase reflex tearing. This may indicate that, lor use in hvdrogel contact lens fitting, additional lime may he required for lens settling due to the potentially irritative nature of the fluid.
Original languageEnglish
Pages (from-to)S151
JournalInvestigative Ophthalmology and Visual Science
Issue number4
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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