Comparison of morphology of bulbar conjunctival cells assessed by impression cytology versus scrape and smear methods

Michael Doughty

Research output: Contribution to journalArticle

Abstract

The aim of this study was to assess the morphology of superficial bulbar conjunctival cells collected by impression cytology or by scraping.
Freshly procured sheep eyes from a slaughterhouse were washed and then conjunctival impression cytology (CIC) samples taken from the nasal aspect of the bulbar conjunctiva on a Biopore Millicell filter unit, or cells scraped from the same location and smeared onto glass slides. After alcohol fixation, all samples were stained with Giemsa. Cell images were obtained by light microscopy at 200× magnification, and subjected to morphometry.
Original languageEnglish
Pages (from-to)973-981
Number of pages9
JournalCurrent Eye Research
Volume39
Issue number10
DOIs
Publication statusPublished - Oct 2014

Keywords

  • conjunctiva
  • impression cytology
  • microscopy

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