Cigarette smoke extract modulates IL-8 release from cystic fibrosis bronchial epithelial cell lines

Mark Williams, Stuart Elborn, Madeleine Ennis

Research output: Contribution to journalArticle

Abstract

Chronic lung disease associated with persistent infection and inflammation is the cause of death of most CF patients. The airways contain excess neutrophils as well as increased levels of pro-inflammatory cytokines. Bronchial epithelial cells play a crucial role in the establishment and continuation of this inflammatory response as they release large amounts of proinflammatory mediators e.g. IL-8 in response to inflammatory stimuli. Cigarette smoke can modulate the release of inflammatory cytokines. The aim of this study was therefore to compare the effects of cigarette smoke extract (CSE) on basal and induced IL-8 secretion from 2 bronchial epithelial cell lines (CFBE41o- and 16HBE14o-). Cells (gift from Dieter C. Gruenert, USF) were pretreated for 4 h with or without 5% CSE. They were then stimulated with LPS from P. aeruginosa (LPS-PA 50 + 100 µg/ml); cytomix 1 (TNF-a 10 ng/ml, IL-1ß 5 ng/ml, LPS-PA 5 µg/ml) or cytomix 2 (TNF-a 10 ng/ml, IL-1ß 10 ng/ml, IFN-¿ 1000 U/ml). Culture supernatants were collected and IL-8 release measured using ELISA (Pelikine). Exposure to CSE significantly reduced the stimulated IL-8 release in all cases in the CFBE41o- cells (cytomix 1: 883.2±143.2 pg/ml, +CSE 575.2±185.4 pg/ml; cytomix 2 3646±520.1 pg/ml, +CSE 1780±304.9 pg/ml; LPS-PA 100 µg/ml 470.5±80.9 pg/ml, +CSE 281.7±64.9 pg/ml; all p
Original languageEnglish
Pages (from-to)287-288
Number of pages2
JournalPediatric Pulmonology
Volume43
Issue numberS31
DOIs
Publication statusPublished - 24 Oct 2008

Keywords

  • cigarette smoke
  • smoking
  • cigarettes
  • cycstic fibrosis

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