Characterisation of gut microbiota of obesity and type 2 diabetes in a rodent model

Khalid S. Ibrahim, Nowara Bourwis, Sharron Dolan, Sue Lang, Janice Spencer, John A. Craft

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
58 Downloads (Pure)


Various studies have suggested that the gut microbiome interacts with the host and may have a significant role in the aetiology of obesity and Type 2 Diabetes (T2D). It was hypothesised that bacterial communities in obesity and T2D differ from control and compromise normal interactions between host and microbiota. Obesity and T2D were developed in rats by feeding a high-fat diet or a high-fat diet plus a single low-dose streptozotocin administration, respectively. The microbiome profiles and their metabolic potentials were established by metagenomic 16S rRNA sequencing and bioinformatics. Taxonomy and predicted metabolism-related genes in obesity and T2D were markedly different from controls and indeed from each other. Diversity was reduced in T2D but not in Obese rats. Factors likely to compromise host intestinal, barrier integrity were found in Obese and T2D rats including predicted, decreased bacterial butyrate production. Capacity to increase energy extraction via ABC-transporters and carbohydrate metabolism were enhanced in Obese and T2D rats. T2D was characterized by increased proinflammatory molecules. While obesity and T2D show distinct differences, results suggest that in both conditions Bacteroides and Blautia species were increased indicating a possible mechanistic link.
Original languageEnglish
Pages (from-to)65-74
Number of pages10
JournalBioscience of Microbiota, Food and Health
Issue number1
Early online date10 Oct 2020
Publication statusPublished - 2021


  • butyrate, inflammatory molecules, microbiome, obesity, Type 2 diabetes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Gastroenterology
  • Food Science
  • Microbiology
  • Immunology


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