Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17a-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts

Joseph Humble; Elaine Hands; Minna Saaristo; Kai  Lindström; Kari K.  Lehtonen; Oihane  Diaz de Cerio; Ibon  Cancio; Gemma  Wilson; John Craft

doi:10.1016/j.chemosphere.2012.11.053

Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17a-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts

Joseph Humble, Elaine Hands, Minna Saaristo, Kai Lindström, Kari K. Lehtonen, Oihane Diaz de Cerio, Ibon Cancio, Gemma Wilson, John Craft

Biological and Biomedical Sciences

Research output: Contribution to journal › Article

Abstract

Oestrogenic endocrine disruptors (OED) pose a significant threat to teleost fish species. The xeno-oestrogen 17a-ethinyloestradiol (EE2) impairs the reproduction of the sand goby (Pomatoschistus minutus), a marine and estuarine teleost, and is known to induce vitellogenin (VTG) and zona radiata protein (ZRP) transcription in males. VTG is the precursor of egg yolk proteins while ZRP forms part of the egg shell, and their inductions are used as biomarkers indicating OED exposure. Multiple forms of VTGs and ZRPs genes are found in teleost species, yet the characteristics of VTG and ZRP in the sand goby are unknown despite their previous use in studies of endocrine disruption and reproduction.

Original language	English
Pages (from-to)	2722–2729
Number of pages	8
Journal	Chemosphere
Volume	90
Issue number	11
Early online date	25 Dec 2012
DOIs	https://doi.org/10.1016/j.chemosphere.2012.11.053
Publication status	Published - Mar 2013

Keywords

sandy goby
oestrogenic endocrine disruptors
teleost

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10.1016/j.chemosphere.2012.11.053

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Humble, J., Hands, E., Saaristo, M., Lindström, K., Lehtonen, K. K., Diaz de Cerio, O., Cancio, I., Wilson, G., & Craft, J. (2013). Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17a-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts. Chemosphere, 90(11), 2722–2729. https://doi.org/10.1016/j.chemosphere.2012.11.053

Humble, Joseph ; Hands, Elaine ; Saaristo, Minna ; Lindström, Kai ; Lehtonen, Kari K. ; Diaz de Cerio, Oihane ; Cancio, Ibon ; Wilson, Gemma ; Craft, John. / Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17a-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts. In: Chemosphere. 2013 ; Vol. 90, No. 11. pp. 2722–2729.

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title = "Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17a-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts",

abstract = "Oestrogenic endocrine disruptors (OED) pose a significant threat to teleost fish species. The xeno-oestrogen 17a-ethinyloestradiol (EE2) impairs the reproduction of the sand goby (Pomatoschistus minutus), a marine and estuarine teleost, and is known to induce vitellogenin (VTG) and zona radiata protein (ZRP) transcription in males. VTG is the precursor of egg yolk proteins while ZRP forms part of the egg shell, and their inductions are used as biomarkers indicating OED exposure. Multiple forms of VTGs and ZRPs genes are found in teleost species, yet the characteristics of VTG and ZRP in the sand goby are unknown despite their previous use in studies of endocrine disruption and reproduction. ",

keywords = "sandy goby, oestrogenic endocrine disruptors, teleost",

author = "Joseph Humble and Elaine Hands and Minna Saaristo and Kai Lindstr{\"o}m and Lehtonen, {Kari K.} and {Diaz de Cerio}, Oihane and Ibon Cancio and Gemma Wilson and John Craft",

year = "2013",

month = mar,

doi = "10.1016/j.chemosphere.2012.11.053",

language = "English",

volume = "90",

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journal = "Chemosphere",

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Humble, J, Hands, E, Saaristo, M, Lindström, K, Lehtonen, KK, Diaz de Cerio, O, Cancio, I, Wilson, G & Craft, J 2013, 'Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17a-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts', Chemosphere, vol. 90, no. 11, pp. 2722–2729. https://doi.org/10.1016/j.chemosphere.2012.11.053

Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17a-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts. / Humble, Joseph; Hands, Elaine; Saaristo, Minna; Lindström, Kai ; Lehtonen, Kari K. ; Diaz de Cerio, Oihane ; Cancio, Ibon ; Wilson, Gemma ; Craft, John.

In: Chemosphere, Vol. 90, No. 11, 03.2013, p. 2722–2729.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17a-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts

AU - Humble, Joseph

AU - Hands, Elaine

AU - Saaristo, Minna

AU - Lindström, Kai

AU - Lehtonen, Kari K.

AU - Diaz de Cerio, Oihane

AU - Cancio, Ibon

AU - Wilson, Gemma

AU - Craft, John

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AB - Oestrogenic endocrine disruptors (OED) pose a significant threat to teleost fish species. The xeno-oestrogen 17a-ethinyloestradiol (EE2) impairs the reproduction of the sand goby (Pomatoschistus minutus), a marine and estuarine teleost, and is known to induce vitellogenin (VTG) and zona radiata protein (ZRP) transcription in males. VTG is the precursor of egg yolk proteins while ZRP forms part of the egg shell, and their inductions are used as biomarkers indicating OED exposure. Multiple forms of VTGs and ZRPs genes are found in teleost species, yet the characteristics of VTG and ZRP in the sand goby are unknown despite their previous use in studies of endocrine disruption and reproduction.

KW - sandy goby

KW - oestrogenic endocrine disruptors

KW - teleost

U2 - 10.1016/j.chemosphere.2012.11.053

DO - 10.1016/j.chemosphere.2012.11.053

M3 - Article

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JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

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Humble J, Hands E, Saaristo M, Lindström K, Lehtonen KK, Diaz de Cerio O et al. Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17a-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts. Chemosphere. 2013 Mar;90(11):2722–2729. https://doi.org/10.1016/j.chemosphere.2012.11.053