Changes in the properties of Bacillus thuringiensis after prolonged culture in a rich medium

M. F. Bizzarri, A. H. Bishop, A. Dinsdale, Niall A. Logan

Research output: Contribution to journalArticle

Abstract

Four strains of B. thuringiensis, which had been isolated in vegetative form from leaf surfaces, were grown for 500 generations in batch culture in a rich medium. One of the strains, S4g, differed from the parent in the following respects: greater cell width; changed plasmid profile; complete loss of ability to produce d-endotoxins; loss of ability to produce ß-exotoxin and disruption of vip3 gene; radically different fatty acid composition; and altered metabolic activity. Two of the other evolved strains (S1g and S6g) showed differences in fatty acid profiles compared with the parents. Genetic finger-printing showed that there were also mutations in the cry genes of two of the evolved strains (S1g and S2g). The d-endotoxins of strain S6g were significantly less toxic to the larvae of Pieris brassica compared with those of the parent and it also differed in the plasmid content.

Original languageEnglish
Pages (from-to)60-69
Number of pages10
JournalJournal of Applied Microbiology
Volume104
Issue number1
DOIs
Publication statusPublished - 1 Jan 2008

Keywords

  • Bacillus thuringiensis
  • bacteriology

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