Cell viability assays for Candida auris

Jason L Brown, Bryn Short, Alicia Ware, Leighann Sherry, Ryan Kean, Gordon Ramage

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

1 Citation (Scopus)

Abstract

Cell viability assays are useful for assessing the efficacy of antifungal therapeutics and disinfection strategies in vitro. In recent years these assays have been fundamental for the testing of conventional and novel therapies against the nosocomial fungal pathogen Candida auris. Here we provide detailed descriptions of methods for assessing cellular viability of Candida auris in vitro, such as metabolic assays (XTT and resazurin), colony-forming unit counting, live/dead quantitative PCR, and fluorescent staining for microscopic analyses.

Original languageEnglish
Title of host publicationCandida auris. Methods in Molecular Biology
EditorsAlexander Lorenz
Place of PublicationNew York
PublisherSpringer Nature
Pages129-153
Number of pages25
Volume2517
ISBN (Electronic)9781071624173
ISBN (Print)9781071624166
DOIs
Publication statusPublished - 9 Jun 2022

Publication series

Name
ISSN (Print)1064-3745

Keywords

  • antifungal agents/pharmacology
  • candida
  • candida auris
  • candidiasis/microbiology
  • cell survival
  • humans
  • microbial sensitivity tests
  • colony-forming units
  • fluorescent staining
  • cell viability
  • resazurin
  • XTT
  • live/dead qPCR

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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