Carbonic anhydrase iii s-glutathionylation is necessary for anti-oxidant activity

P. Roy; M. Ireland; S. Roy; J. Craft; C. Bartholomew

doi:10.4236/ym.2018.24026

Carbonic anhydrase iii s-glutathionylation is necessary for anti-oxidant activity

P. Roy, M. Ireland, S. Roy, J. Craft, C. Bartholomew

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Abstract

Carbonic anhydrase isozyme CA3 protects cells against oxidative stress. Ectopic expression of murine Ca3, but not Ca2, protects proto-oncogene Evi1 expressing Rat1 fibroblast cells (ca3low) against hydrogen peroxide (H2O2) induced stress. Ca3 is S-glutathionylated via glutathione adducts with cysteines 181 and 186. Substitution of both Ca3 cysteines with serine fails to protect cells from oxidative stress. Insertion of cysteine at 181 and 186 in Ca2 is insufficient for conferring efficient anti-oxidant activity. This shows for the first time that S-glutathionylation of cys181 and cys186 residues is required for Ca3 anti-oxidant activity but that additional factors are also required.

Original language	English
Article number	89310
Pages (from-to)	244-254
Number of pages	11
Journal	Yangtze Medicine
Volume	2
Issue number	4
DOIs	https://doi.org/10.4236/ym.2018.24026
Publication status	Published - 21 Dec 2018

Keywords

CA3
CAIII
Carbonic anhydrase III
S-glutathionylation
apoptosis
anti-oxidant

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10.4236/ym.2018.24026

Roy, P. et al (2018) Carbonic Anhydrase III S-Glutathionylation Is Necessary for Anti-Oxidant Activity
Copyright © 2018 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/
Final published version, 762 KBLicence: CC BY

Cite this

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abstract = "Carbonic anhydrase isozyme CA3 protects cells against oxidative stress. Ectopic expression of murine Ca3, but not Ca2, protects proto-oncogene Evi1 expressing Rat1 fibroblast cells (ca3low) against hydrogen peroxide (H2O2) induced stress. Ca3 is S-glutathionylated via glutathione adducts with cysteines 181 and 186. Substitution of both Ca3 cysteines with serine fails to protect cells from oxidative stress. Insertion of cysteine at 181 and 186 in Ca2 is insufficient for conferring efficient anti-oxidant activity. This shows for the first time that S-glutathionylation of cys181 and cys186 residues is required for Ca3 anti-oxidant activity but that additional factors are also required.",

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author = "P. Roy and M. Ireland and S. Roy and J. Craft and C. Bartholomew",

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AU - Roy, P.

AU - Ireland, M.

AU - Roy, S.

AU - Craft, J.

AU - Bartholomew, C.

N1 - Acceptance from webpage Published version title: Carbonic Anhydrase III S-Glutathionylation Is Necessary for Anti-Oxidant Activity

PY - 2018/12/21

Y1 - 2018/12/21

N2 - Carbonic anhydrase isozyme CA3 protects cells against oxidative stress. Ectopic expression of murine Ca3, but not Ca2, protects proto-oncogene Evi1 expressing Rat1 fibroblast cells (ca3low) against hydrogen peroxide (H2O2) induced stress. Ca3 is S-glutathionylated via glutathione adducts with cysteines 181 and 186. Substitution of both Ca3 cysteines with serine fails to protect cells from oxidative stress. Insertion of cysteine at 181 and 186 in Ca2 is insufficient for conferring efficient anti-oxidant activity. This shows for the first time that S-glutathionylation of cys181 and cys186 residues is required for Ca3 anti-oxidant activity but that additional factors are also required.

AB - Carbonic anhydrase isozyme CA3 protects cells against oxidative stress. Ectopic expression of murine Ca3, but not Ca2, protects proto-oncogene Evi1 expressing Rat1 fibroblast cells (ca3low) against hydrogen peroxide (H2O2) induced stress. Ca3 is S-glutathionylated via glutathione adducts with cysteines 181 and 186. Substitution of both Ca3 cysteines with serine fails to protect cells from oxidative stress. Insertion of cysteine at 181 and 186 in Ca2 is insufficient for conferring efficient anti-oxidant activity. This shows for the first time that S-glutathionylation of cys181 and cys186 residues is required for Ca3 anti-oxidant activity but that additional factors are also required.

KW - CA3

KW - CAIII

KW - Carbonic anhydrase III

KW - S-glutathionylation

KW - apoptosis

KW - anti-oxidant

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DO - 10.4236/ym.2018.24026

M3 - Article

SN - 2475-7349

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JO - Yangtze Medicine

JF - Yangtze Medicine

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M1 - 89310

ER -

Carbonic anhydrase iii s-glutathionylation is necessary for anti-oxidant activity

Abstract

Keywords

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