Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica

R. N. Allan, L. Lebbe, J. Heyrman, Paul De Vos, C. J. Buchanan, Niall A. Logan

Research output: Contribution to journalArticle

Abstract

Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of Brevibacillus and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of Aneurinibacillus. Brevibacillus strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and Aneurinibacillus strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that L-glutamic acid, although not essential for growth, was utilized by both species. The Brevibacillus species possessed an uptake system specific for L-glutamic acid, whereas the Aneurinibacillus species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K+ antiport system and similar energy systems for the uptake of L-glutamic acid. The rate of uptake by the Brevibacillus species type strain was 20-fold greater than that shown by the Aneurinibacillus species type strain. The names Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657T (=LMG 22481T=CIP 108307T) and Logan B-1599T (LMG 22483T= CIP 108308T), respectively.

Original languageEnglish
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume55
DOIs
Publication statusPublished - May 2005

Fingerprint

Aneurinibacillus
Brevibacillus
Antarctica
Soil
cryptogams
new species
Glutamic Acid
glutamic acid
uptake mechanisms
soil
Endospore-Forming Bacteria
Ion Transport
acid
endospores
metabolic studies
rRNA Genes
Names
Polyacrylamide Gel Electrophoresis
land
Amino Acids

Keywords

  • Antarctica
  • thermoacidophiles
  • Brevibacillus
  • Aneurinibacillus

Cite this

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title = "Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica",
abstract = "Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of Brevibacillus and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of Aneurinibacillus. Brevibacillus strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and Aneurinibacillus strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that L-glutamic acid, although not essential for growth, was utilized by both species. The Brevibacillus species possessed an uptake system specific for L-glutamic acid, whereas the Aneurinibacillus species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K+ antiport system and similar energy systems for the uptake of L-glutamic acid. The rate of uptake by the Brevibacillus species type strain was 20-fold greater than that shown by the Aneurinibacillus species type strain. The names Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657T (=LMG 22481T=CIP 108307T) and Logan B-1599T (LMG 22483T= CIP 108308T), respectively.",
keywords = "Antarctica, thermoacidophiles, Brevibacillus, Aneurinibacillus",
author = "Allan, {R. N.} and L. Lebbe and J. Heyrman and {De Vos}, Paul and Buchanan, {C. J.} and Logan, {Niall A.}",
note = "Originally published in: International Journal of Systematic and Evolutionary Microbiology (2004), 155 (6), pp.483-490. Publisher version not permitted in repositories.",
year = "2005",
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language = "English",
volume = "55",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
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Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica. / Allan, R. N.; Lebbe, L.; Heyrman, J.; De Vos, Paul; Buchanan, C. J.; Logan, Niall A.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 55, 05.2005.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica

AU - Allan, R. N.

AU - Lebbe, L.

AU - Heyrman, J.

AU - De Vos, Paul

AU - Buchanan, C. J.

AU - Logan, Niall A.

N1 - Originally published in: International Journal of Systematic and Evolutionary Microbiology (2004), 155 (6), pp.483-490. Publisher version not permitted in repositories.

PY - 2005/5

Y1 - 2005/5

N2 - Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of Brevibacillus and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of Aneurinibacillus. Brevibacillus strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and Aneurinibacillus strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that L-glutamic acid, although not essential for growth, was utilized by both species. The Brevibacillus species possessed an uptake system specific for L-glutamic acid, whereas the Aneurinibacillus species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K+ antiport system and similar energy systems for the uptake of L-glutamic acid. The rate of uptake by the Brevibacillus species type strain was 20-fold greater than that shown by the Aneurinibacillus species type strain. The names Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657T (=LMG 22481T=CIP 108307T) and Logan B-1599T (LMG 22483T= CIP 108308T), respectively.

AB - Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of Brevibacillus and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of Aneurinibacillus. Brevibacillus strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and Aneurinibacillus strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that L-glutamic acid, although not essential for growth, was utilized by both species. The Brevibacillus species possessed an uptake system specific for L-glutamic acid, whereas the Aneurinibacillus species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K+ antiport system and similar energy systems for the uptake of L-glutamic acid. The rate of uptake by the Brevibacillus species type strain was 20-fold greater than that shown by the Aneurinibacillus species type strain. The names Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657T (=LMG 22481T=CIP 108307T) and Logan B-1599T (LMG 22483T= CIP 108308T), respectively.

KW - Antarctica

KW - thermoacidophiles

KW - Brevibacillus

KW - Aneurinibacillus

U2 - 10.1099/ijs.0.63397-0

DO - 10.1099/ijs.0.63397-0

M3 - Article

VL - 55

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

ER -