BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells

M. Düfer; Y. Neye; K. Düfer; P. Krippeit-Drews; A. Hennige; Hélène Widmer; H. McClafferty; M. J. Shipston

doi:10.1007/s00125-010-1936-0

BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells

M. Düfer, Y. Neye, K. Düfer, P. Krippeit-Drews, A. Hennige, Hélène Widmer, H. McClafferty, M. J. Shipston

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Evidence is accumulating that Ca²⁺-regulated K⁺ (K_Ca) channels are important for beta cell function. We used BK channel knockout (BK-KO) mice to examine the role of these K_Ca channels for glucose homeostasis, beta cell function and viability. Glucose and insulin tolerance were tested with male wild-type and BK-KO mice. BK channels were detected by single-cell RT-PCR, cytosolic Ca²⁺ concentration ([Ca²⁺]_c) by fura-2 fluorescence, and insulin secretion by radioimmunoassay. Electrophysiology was performed with the patch-clamp technique. Apoptosis was detected via caspase 3 or TUNEL assay.

Original language	English
Pages (from-to)	423-432
Number of pages	10
Journal	Diabetologia
Volume	54
Issue number	2
DOIs	https://doi.org/10.1007/s00125-010-1936-0
Publication status	Published - 1 Jan 2010

Keywords

apoptosis
insulin
glucose homeostasis

Documents and Links

10.1007/s00125-010-1936-0

Cite this

@article{d7506efe39004d7fb5d44efdbd7317d3,

title = "BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells",

abstract = "Evidence is accumulating that Ca2+-regulated K+ (KCa) channels are important for beta cell function. We used BK channel knockout (BK-KO) mice to examine the role of these KCa channels for glucose homeostasis, beta cell function and viability. Glucose and insulin tolerance were tested with male wild-type and BK-KO mice. BK channels were detected by single-cell RT-PCR, cytosolic Ca2+ concentration ([Ca2+]c) by fura-2 fluorescence, and insulin secretion by radioimmunoassay. Electrophysiology was performed with the patch-clamp technique. Apoptosis was detected via caspase 3 or TUNEL assay.",

keywords = "apoptosis, insulin, glucose homeostasis",

author = "M. D{\"u}fer and Y. Neye and K. D{\"u}fer and P. Krippeit-Drews and A. Hennige and H{\'e}l{\`e}ne Widmer and H. McClafferty and Shipston, {M. J.}",

note = "<p>Originally published in: Diabetologia (2010), 54 (2), pp.423-432.</p>",

year = "2010",

month = jan,

day = "1",

doi = "10.1007/s00125-010-1936-0",

language = "English",

volume = "54",

pages = "423--432",

journal = "Diabetologia",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "2",

}

TY - JOUR

T1 - BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells

AU - Düfer, M.

AU - Neye, Y.

AU - Düfer, K.

AU - Krippeit-Drews, P.

AU - Hennige, A.

AU - Widmer, Hélène

AU - McClafferty, H.

AU - Shipston, M. J.

N1 - <p>Originally published in: Diabetologia (2010), 54 (2), pp.423-432.</p>

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Evidence is accumulating that Ca2+-regulated K+ (KCa) channels are important for beta cell function. We used BK channel knockout (BK-KO) mice to examine the role of these KCa channels for glucose homeostasis, beta cell function and viability. Glucose and insulin tolerance were tested with male wild-type and BK-KO mice. BK channels were detected by single-cell RT-PCR, cytosolic Ca2+ concentration ([Ca2+]c) by fura-2 fluorescence, and insulin secretion by radioimmunoassay. Electrophysiology was performed with the patch-clamp technique. Apoptosis was detected via caspase 3 or TUNEL assay.

AB - Evidence is accumulating that Ca2+-regulated K+ (KCa) channels are important for beta cell function. We used BK channel knockout (BK-KO) mice to examine the role of these KCa channels for glucose homeostasis, beta cell function and viability. Glucose and insulin tolerance were tested with male wild-type and BK-KO mice. BK channels were detected by single-cell RT-PCR, cytosolic Ca2+ concentration ([Ca2+]c) by fura-2 fluorescence, and insulin secretion by radioimmunoassay. Electrophysiology was performed with the patch-clamp technique. Apoptosis was detected via caspase 3 or TUNEL assay.

KW - apoptosis

KW - insulin

KW - glucose homeostasis

U2 - 10.1007/s00125-010-1936-0

DO - 10.1007/s00125-010-1936-0

M3 - Article

VL - 54

SP - 423

EP - 432

JO - Diabetologia

JF - Diabetologia

IS - 2

ER -

BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells

Abstract

Keywords

Documents and Links

Fingerprint

Cite this