BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells

M. Düfer, Y. Neye, K. Düfer, P. Krippeit-Drews, A. Hennige, Hélène Widmer, H. McClafferty, M. J. Shipston

Research output: Contribution to journalArticle

Abstract

Evidence is accumulating that Ca2+-regulated K+ (KCa) channels are important for beta cell function. We used BK channel knockout (BK-KO) mice to examine the role of these KCa channels for glucose homeostasis, beta cell function and viability. Glucose and insulin tolerance were tested with male wild-type and BK-KO mice. BK channels were detected by single-cell RT-PCR, cytosolic Ca2+ concentration ([Ca2+]c) by fura-2 fluorescence, and insulin secretion by radioimmunoassay. Electrophysiology was performed with the patch-clamp technique. Apoptosis was detected via caspase 3 or TUNEL assay.

Original languageEnglish
Pages (from-to)423-432
Number of pages10
JournalDiabetologia
Volume54
Issue number2
DOIs
Publication statusPublished - 1 Jan 2010

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Large-Conductance Calcium-Activated Potassium Channels
Insulin-Secreting Cells
Cell Survival
Homeostasis
Glucose
Knockout Mice
Insulin
Fura-2
Electrophysiology
In Situ Nick-End Labeling
Patch-Clamp Techniques
Caspase 3
Radioimmunoassay
Fluorescence
Apoptosis
Polymerase Chain Reaction

Keywords

  • apoptosis
  • insulin
  • glucose homeostasis

Cite this

Düfer, M., Neye, Y., Düfer, K., Krippeit-Drews, P., Hennige, A., Widmer, H., ... Shipston, M. J. (2010). BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells. Diabetologia, 54(2), 423-432. https://doi.org/10.1007/s00125-010-1936-0
Düfer, M. ; Neye, Y. ; Düfer, K. ; Krippeit-Drews, P. ; Hennige, A. ; Widmer, Hélène ; McClafferty, H. ; Shipston, M. J. / BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells. In: Diabetologia. 2010 ; Vol. 54, No. 2. pp. 423-432.
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Düfer, M, Neye, Y, Düfer, K, Krippeit-Drews, P, Hennige, A, Widmer, H, McClafferty, H & Shipston, MJ 2010, 'BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells', Diabetologia, vol. 54, no. 2, pp. 423-432. https://doi.org/10.1007/s00125-010-1936-0

BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells. / Düfer, M.; Neye, Y.; Düfer, K.; Krippeit-Drews, P.; Hennige, A.; Widmer, Hélène; McClafferty, H.; Shipston, M. J.

In: Diabetologia, Vol. 54, No. 2, 01.01.2010, p. 423-432.

Research output: Contribution to journalArticle

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AU - Düfer, M.

AU - Neye, Y.

AU - Düfer, K.

AU - Krippeit-Drews, P.

AU - Hennige, A.

AU - Widmer, Hélène

AU - McClafferty, H.

AU - Shipston, M. J.

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AB - Evidence is accumulating that Ca2+-regulated K+ (KCa) channels are important for beta cell function. We used BK channel knockout (BK-KO) mice to examine the role of these KCa channels for glucose homeostasis, beta cell function and viability. Glucose and insulin tolerance were tested with male wild-type and BK-KO mice. BK channels were detected by single-cell RT-PCR, cytosolic Ca2+ concentration ([Ca2+]c) by fura-2 fluorescence, and insulin secretion by radioimmunoassay. Electrophysiology was performed with the patch-clamp technique. Apoptosis was detected via caspase 3 or TUNEL assay.

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Düfer M, Neye Y, Düfer K, Krippeit-Drews P, Hennige A, Widmer H et al. BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells. Diabetologia. 2010 Jan 1;54(2):423-432. https://doi.org/10.1007/s00125-010-1936-0