BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells

M. Düfer, Y. Neye, K. Düfer, P. Krippeit-Drews, A. Hennige, Hélène Widmer, H. McClafferty, M. J. Shipston

Research output: Contribution to journalArticle

Abstract

Evidence is accumulating that Ca2+-regulated K+ (KCa) channels are important for beta cell function. We used BK channel knockout (BK-KO) mice to examine the role of these KCa channels for glucose homeostasis, beta cell function and viability. Glucose and insulin tolerance were tested with male wild-type and BK-KO mice. BK channels were detected by single-cell RT-PCR, cytosolic Ca2+ concentration ([Ca2+]c) by fura-2 fluorescence, and insulin secretion by radioimmunoassay. Electrophysiology was performed with the patch-clamp technique. Apoptosis was detected via caspase 3 or TUNEL assay.

Original languageEnglish
Pages (from-to)423-432
Number of pages10
JournalDiabetologia
Volume54
Issue number2
DOIs
Publication statusPublished - 1 Jan 2010

Keywords

  • apoptosis
  • insulin
  • glucose homeostasis

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    Düfer, M., Neye, Y., Düfer, K., Krippeit-Drews, P., Hennige, A., Widmer, H., McClafferty, H., & Shipston, M. J. (2010). BK channels affect glucose homeostasis and cell viability of muring pancreatic beta cells. Diabetologia, 54(2), 423-432. https://doi.org/10.1007/s00125-010-1936-0