Biosorption of metals (Cu2+, Zn2+) and anions (F-, H2PO4-) by viable and autoclaved cells of the Gram-negative bacterium Shewanella putrefaciens

Natalia Chubar, Thilo Behrends, Philippe Van Cappellen

    Research output: Contribution to journalArticle

    Abstract

    Microbial biomass represents a potentially cost-effective sorbent for water treatment applications. High sorption capacities for both cations and anions are demonstrated here for viable and autoclaved cell suspensions of the Gram-negative bacterium Shewanella putrefaciens. FTIR absorption spectra and pHdependent zeta-potentials are similar for the viable and killed bacterial cells. Potentiometric titrations, however, reveal a two to three times higher OH- buffering capacity for the living cells. The Cu2+ sorption capacity of the viable cells is also about twice that of the autoclaved cells. Sorption of fluoride and phosphate is not pH-dependent, although an initial addition of acid or base was needed to activate the anion binding sites. Uptake of fluoride is comparable for viable and killed cells.
    Original languageEnglish
    Pages (from-to)126-133
    Number of pages8
    JournalColloids and Surfaces B: Biointerfaces
    Volume65
    Issue number1
    DOIs
    Publication statusPublished - Aug 2008

    Fingerprint

    Shewanella putrefaciens
    Biosorption
    Gram-Negative Bacteria
    bacteria
    Anions
    Sorption
    Bacteria
    Negative ions
    Metals
    anions
    Fluorides
    sorption
    cells
    metals
    Binding sites
    Zeta potential
    fluorides
    Sorbents
    Titration
    Water treatment

    Keywords

    • biosorption
    • bio-engineering
    • Shewanella putrefciens
    • bioremediation

    Cite this

    @article{af4455cf7add4274a1a1491f5b467049,
    title = "Biosorption of metals (Cu2+, Zn2+) and anions (F-, H2PO4-) by viable and autoclaved cells of the Gram-negative bacterium Shewanella putrefaciens",
    abstract = "Microbial biomass represents a potentially cost-effective sorbent for water treatment applications. High sorption capacities for both cations and anions are demonstrated here for viable and autoclaved cell suspensions of the Gram-negative bacterium Shewanella putrefaciens. FTIR absorption spectra and pHdependent zeta-potentials are similar for the viable and killed bacterial cells. Potentiometric titrations, however, reveal a two to three times higher OH- buffering capacity for the living cells. The Cu2+ sorption capacity of the viable cells is also about twice that of the autoclaved cells. Sorption of fluoride and phosphate is not pH-dependent, although an initial addition of acid or base was needed to activate the anion binding sites. Uptake of fluoride is comparable for viable and killed cells.",
    keywords = "biosorption , bio-engineering, Shewanella putrefciens, bioremediation",
    author = "Natalia Chubar and Thilo Behrends and {Van Cappellen}, Philippe",
    year = "2008",
    month = "8",
    doi = "10.1016/j.colsurfb.2008.03.006",
    language = "English",
    volume = "65",
    pages = "126--133",
    journal = "Colloids and Surfaces B: Biointerfaces",
    issn = "0927-7765",
    publisher = "Elsevier B.V.",
    number = "1",

    }

    Biosorption of metals (Cu2+, Zn2+) and anions (F-, H2PO4-) by viable and autoclaved cells of the Gram-negative bacterium Shewanella putrefaciens. / Chubar, Natalia; Behrends, Thilo; Van Cappellen, Philippe.

    In: Colloids and Surfaces B: Biointerfaces, Vol. 65, No. 1, 08.2008, p. 126-133.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Biosorption of metals (Cu2+, Zn2+) and anions (F-, H2PO4-) by viable and autoclaved cells of the Gram-negative bacterium Shewanella putrefaciens

    AU - Chubar, Natalia

    AU - Behrends, Thilo

    AU - Van Cappellen, Philippe

    PY - 2008/8

    Y1 - 2008/8

    N2 - Microbial biomass represents a potentially cost-effective sorbent for water treatment applications. High sorption capacities for both cations and anions are demonstrated here for viable and autoclaved cell suspensions of the Gram-negative bacterium Shewanella putrefaciens. FTIR absorption spectra and pHdependent zeta-potentials are similar for the viable and killed bacterial cells. Potentiometric titrations, however, reveal a two to three times higher OH- buffering capacity for the living cells. The Cu2+ sorption capacity of the viable cells is also about twice that of the autoclaved cells. Sorption of fluoride and phosphate is not pH-dependent, although an initial addition of acid or base was needed to activate the anion binding sites. Uptake of fluoride is comparable for viable and killed cells.

    AB - Microbial biomass represents a potentially cost-effective sorbent for water treatment applications. High sorption capacities for both cations and anions are demonstrated here for viable and autoclaved cell suspensions of the Gram-negative bacterium Shewanella putrefaciens. FTIR absorption spectra and pHdependent zeta-potentials are similar for the viable and killed bacterial cells. Potentiometric titrations, however, reveal a two to three times higher OH- buffering capacity for the living cells. The Cu2+ sorption capacity of the viable cells is also about twice that of the autoclaved cells. Sorption of fluoride and phosphate is not pH-dependent, although an initial addition of acid or base was needed to activate the anion binding sites. Uptake of fluoride is comparable for viable and killed cells.

    KW - biosorption

    KW - bio-engineering

    KW - Shewanella putrefciens

    KW - bioremediation

    U2 - 10.1016/j.colsurfb.2008.03.006

    DO - 10.1016/j.colsurfb.2008.03.006

    M3 - Article

    VL - 65

    SP - 126

    EP - 133

    JO - Colloids and Surfaces B: Biointerfaces

    JF - Colloids and Surfaces B: Biointerfaces

    SN - 0927-7765

    IS - 1

    ER -