Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells

Jason L Brown, William Johnston, Christopher Delaney, Ranjith Rajendran, John Butcher, Shaz Khan, David Bradshaw, Gordon Ramage, Shauna Culshaw

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Abstract

The gingival epithelium is a physical and immunological barrier to the microbiota of the oral cavity, which interact through soluble mediators with the immune cells that patrol the tissue at the gingival epithelium. We sought to develop a three-dimensional gingivae-biofilm interface model using a commercially available gingival epithelium to study the tissue inflammatory response to oral biofilms associated with “health”, “gingivitis” and “periodontitis.”. These biofilms were developed by sequential addition of microorganisms to mimic the formation of supra- and sub-gingival plaque in vivo. Secondly, to mimic the interactions between gingival epithelium and immune cells in vivo, we integrated peripheral blood mononuclear cells and CD14+ monocytes into our three-dimensional model and were able to assess the inflammatory response in the immune cells cultured with and without gingival epithelium. We describe a differential inflammatory response in immune cells cultured with epithelial tissue, and more so following incubation with epithelium stimulated by “gingivitis-associated” biofilm. These results suggest that gingival epithelium-derived soluble mediators may control the inflammatory status of immune cells in vitro, and therefore targeting of the epithelial response may offer novel therapies. This multi-cellular interface model, both of microbial and host origin, offers a robust in vitro platform to investigate host-pathogens at the epithelial surface.
Original languageEnglish
Article number15779
Number of pages14
JournalScientific Reports
DOIs
Publication statusPublished - 31 Oct 2019

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Keywords

  • oral microbiology
  • biofilm

Cite this

Brown, J. L., Johnston, W., Delaney, C., Rajendran, R., Butcher, J., Khan, S., Bradshaw, D., Ramage, G., & Culshaw, S. (2019). Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells. Scientific Reports, [15779]. https://doi.org/10.1038/s41598-019-52115-7