Assessment of the reliability of endothelial cell-density estimates in the presence of pseudoguttata

Michael Doughty, Sven Jonuscheit, Norman Button

Research output: Contribution to journalArticle

Abstract

The purpose of this work is to assess the reliability of endothelial cell-density (ECD) estimates in corneas with different severity pseudoguttata.Specular microscopy was undertaken on grade 1, 2, or 3 pseudoguttata patients and age-matched controls aged 52-83 years. On high magnification prints of central cornea, areas of complete cells (all sides visible) and partial 'cells' (one or more sides obscured) were measured manually. Sets of 45 complete cells were selected, as well as 75 cells that were a mixture of complete and partial cells on guttate endothelia. ECD was calculated by a progressive averaging technique.

Original languageEnglish
Pages (from-to)111-121
Number of pages11
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume250
Issue number1
Early online date13 Sep 2011
DOIs
Publication statusPublished - 2012

Keywords

  • vision science
  • endothelial cell-density
  • cornea
  • ophthalmology

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