Assessment of size and nucleo-cytoplasmic characteristics of the squamous cells of the corneal epithelium

Michael Doughty

Research output: Contribution to journalArticle

Abstract

The aim was to objectively assess size, nucleus and nucleo-cytoplasmic ratio features of squamous cells from the corneal epithelium. The corneas of recent post-mortem sheep eyes were either glutaraldehyde-fixed for transmission electron microscopy or impression cytology samples taken, glutaraldehyde-fixed and stained with Giemsa. From the specimens for impression cytology, a representative region was photographed from 12 different samples taken from the central region and 16 different samples taken from mid-peripheral regions of the corneal epithelium. Images were subjected to morphometry after overlays were generated.

Original languageEnglish
Pages (from-to)218-223
Number of pages6
JournalClinical and Experimental Optometry
Volume98
Issue number3
DOIs
Publication statusPublished - May 2015

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Corneal Epithelium
Glutaral
Cell Biology
Epithelial Cells
Transmission Electron Microscopy
Cornea
Sheep

Keywords

  • corneal epithelium
  • squamous cells
  • morphometry

Cite this

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Assessment of size and nucleo-cytoplasmic characteristics of the squamous cells of the corneal epithelium. / Doughty, Michael.

In: Clinical and Experimental Optometry, Vol. 98, No. 3, 05.2015, p. 218-223.

Research output: Contribution to journalArticle

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