Assessment of goblet cell orifice distribution across the rabbit bulbar conjunctiva based on numerical density and nearest neighbors analysis

Michael Doughty

Research output: Contribution to journalArticle

Abstract

The aim of this study was to assess density and spatial distribution of the goblet cell orifices at the surface of the rabbit bulbar conjunctiva as an indicator of functional activity. Specimens of the superior or inferior bulbar conjunctiva from six healthy young adult (2¿kg) pigmented rabbits were obtained using a special preparation technique by which the conjunctiva was carefully stretched out during fixation with buffered glutaraldehyde. The apical surface of the specimens was examined by scanning electron microscopy. From high magnification prints, the areas and dimensions of 32-49 orifices/image were measured. In addition, the centre-to-centre spacing and spatial distribution of the orifices were assessed using a nearest neighbors principle.
Original languageEnglish
Pages (from-to)237-251
Number of pages15
JournalCurrent Eye Research
Volume38
Issue number2
DOIs
Publication statusPublished - Feb 2013

Keywords

  • vision sciences
  • visual neuroscience
  • bulbar conjunctiva
  • scanning electron microscopy

Fingerprint Dive into the research topics of 'Assessment of goblet cell orifice distribution across the rabbit bulbar conjunctiva based on numerical density and nearest neighbors analysis'. Together they form a unique fingerprint.

  • Cite this