Assessment of consistency to assignment of severe (grade 3) squamous metaplasia to human bulbar conjunctiva impression cytology cell samples

Michael Doughty

Research output: Contribution to journalArticle

Abstract

Nucleus-to-cytoplasm (N:C) characteristics of published images of human bulbar conjunctival cells designated as showing severe (grade 3) squamous metaplasia were retrospectively assessed. From publications over a 40-year period, measurements were made from images of cell and nucleus size (based on areas and dimensions) and four different calculations were made for nucleo-cytoplasmic (N:C) ratios. From 54 published images, the mean nucleus-to-cytoplasm area ratio (NU/CYT AREA ratio) was 0.145 +/- 0.077 (range 0.052 to 0.346), compared to two different reference set values of 0.069 +/- 0.017 and 0.080 +/- 0.021. Similarly, a nucleus-to-cytoplasm length ratio (as LNLONG) was 0.308 +/- 0.080 (range 0.191 to 0.475) compared to reference values of 0.226 +/- 0.032 and 0.236 +/- 0.034.
Original languageEnglish
Pages (from-to)284-297
Number of pages14
JournalOcular Surface
Volume13
Issue number4
Early online date9 Jul 2014
DOIs
Publication statusPublished - Oct 2015

Keywords

  • cytology
  • vision sciences
  • squamous metaplasia

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