The aim of this research was to assess the agreement of the 'polygonal' variable frame cell count option on a confocal microscope after keratoplasty, with planimetry as the reference method. MethodsOne hundred clear corneal grafts of 83 patients attending the cornea clinic at Gartnavel General Hospital in Glasgow underwent slit-scanning in vivoconfocal microscopy. Endothelial cell images were assessed with the Nidek Advanced Vision Information System (NAVIS), using the polygonal variable frame and the manual fixed-frame methods. Planimetry was used as the reference. The agreement between methods was assessed by Bland-Altman analysis.
- vision sciences
- corneal transplants
- confocal microscopy
Jonuscheit, S., Doughty, M., & Ramaesh, K. (2012). Assessment of a variable frame (polygonal) method to estimate corneal endothelial cell counts after corneal transplantation. Eye, 26(6), 803-809. https://doi.org/10.1038/eye.2012.42