Anti‑inflammatory activities of Gardenia jasminoides extracts in retinal pigment epithelial cells and zebrafish embryos

Jianrong Chen, Gabriel Mbuta Tchivelekete, Xinzhi Zhou, Weizhuo Tang, Fang Liu, Minzhuo Liu, Chenxi Zhao, Xinhua Shu*, Zhihong Zeng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Age‑related macular degeneration (AMD) is the most common cause of visual impairment in developed countries. Inflammation serves a critical role in the pathogenesis of AMD. Gardenia jasminoides is found in several regions of China and is traditionally used as an organic yellow dye but has also been widely used as a therapeutic agent in numerous diseases, including inflammation, depression, hepatic and vascular disorders, which may reflect the variability of functional compounds that are present in Gardenia jasminoides extracts (GJE). To investigate the therapeutic potential of GJE for AMD, ARPE‑19 cells were treated with lipopolysaccharide (LPS) or LPS plus GJE. GJE significantly decreased LPS‑induced expression of proinflammatory cytokines, including IL‑1β, IL‑6 and TNF‑α. In the in vivo study, GJE inhibited CuSO4‑induced migration of primitive macrophages to the lateral line in zebrafish embryos. GJE also attenuated expression of cytokines (IL‑1β, IL‑6 and TNF‑α), NFKB activating protein (nkap) and TLR4 in ARPE‑19 cells. The results of the present study demonstrated the anti‑inflammatory potential of GJE in vitro and in vivo, and suggested GJE as a therapeutic candidate for AMD.
Original languageEnglish
Article number700
Pages (from-to)1-9
Number of pages9
JournalExperimental and Therapeutic Medicine
Volume22
Issue number1
Early online date2 May 2021
DOIs
Publication statusPublished - Jul 2021

Keywords

  • Gardenia jasminoides extracts
  • retinal pigment epithelial cells
  • zebrafish embryos

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