An in vitro investigation of photodynamic efficacy of FosPeg® on human colon cancer cells

Ricky W.K. Wu; Ellie S.M. Chu; Zheng Huang; Malini C. Olivo; David C.W. Ip; Christine M.N. Yow

doi:10.1142/S1793545815500273

An in vitro investigation of photodynamic efficacy of FosPeg® on human colon cancer cells

Ricky W.K. Wu, Ellie S.M. Chu, Zheng Huang^*, Malini C. Olivo, David C.W. Ip, Christine M.N. Yow^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

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Abstract

Photodynamic therapy (PDT) is a novel therapeutic approach for combating various cancers. PDT involves the administration of a photosensitizer which generates singlet oxygen after light activation. FosPeg ® is the liposomal formulation of mTHPC. In this in vitro study, the photodynamic efficacy of FosPeg ® on a human colon cancer cell line (HT29) was investigated via studying the cellular uptake of FosPeg ®, FosPeg ® PDT mediated photocytotoxicity and the cell death mechanism were triggered. FosPeg ® PDT demonstrated its antitumor effect in a drug and light dose-dependent manner in HT-29 cells. Lethal dose (LD₅₀) was achieved with 0.4 μg/mL of drug and 3 J/cm^-2 of light dose. FosPeg ® PDT triggered apoptotic cell death via activating caspase cascade and regulating cell cycle progression. In conclusion, FosPeg ®-PDT is an effective measure to combat human colon cancer cells.

Original language	English
Article number	1550027
Number of pages	8
Journal	Journal of Innovative Optical Health Sciences
Volume	8
Issue number	5
Early online date	28 Jan 2015
DOIs	https://doi.org/10.1142/S1793545815500273
Publication status	Published - Sept 2015
Externally published	Yes

Keywords

apoptosis
caspase-3
FosPeg ®
HT-29

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Medicine (miscellaneous)
Atomic and Molecular Physics, and Optics
Biomedical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1142/S1793545815500273

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Final published version, 488 KBLicence: CC BY

Cite this

@article{e7032a7ed7154d9b95eb3ff7df178e69,

title = "An in vitro investigation of photodynamic efficacy of FosPeg{\textregistered} on human colon cancer cells",

abstract = "Photodynamic therapy (PDT) is a novel therapeutic approach for combating various cancers. PDT involves the administration of a photosensitizer which generates singlet oxygen after light activation. FosPeg {\textregistered} is the liposomal formulation of mTHPC. In this in vitro study, the photodynamic efficacy of FosPeg {\textregistered} on a human colon cancer cell line (HT29) was investigated via studying the cellular uptake of FosPeg {\textregistered}, FosPeg {\textregistered} PDT mediated photocytotoxicity and the cell death mechanism were triggered. FosPeg {\textregistered} PDT demonstrated its antitumor effect in a drug and light dose-dependent manner in HT-29 cells. Lethal dose (LD50) was achieved with 0.4 μg/mL of drug and 3 J/cm-2 of light dose. FosPeg {\textregistered} PDT triggered apoptotic cell death via activating caspase cascade and regulating cell cycle progression. In conclusion, FosPeg {\textregistered}-PDT is an effective measure to combat human colon cancer cells.",

keywords = "apoptosis, caspase-3, FosPeg {\textregistered}, HT-29",

author = "Wu, {Ricky W.K.} and Chu, {Ellie S.M.} and Zheng Huang and Olivo, {Malini C.} and Ip, {David C.W.} and Yow, {Christine M.N.}",

note = "Funding Information: We thank Biolitec for generously providing the photosensitizer FosPegr. This study was supported by The Hong Kong Polytechnic University Central Research Grant (G-YJ67 and G-U737).",

year = "2015",

month = sep,

doi = "10.1142/S1793545815500273",

language = "English",

volume = "8",

journal = "Journal of Innovative Optical Health Sciences",

issn = "1793-5458",

publisher = "World Scientific",

number = "5",

}

TY - JOUR

T1 - An in vitro investigation of photodynamic efficacy of FosPeg® on human colon cancer cells

AU - Wu, Ricky W.K.

AU - Chu, Ellie S.M.

AU - Huang, Zheng

AU - Olivo, Malini C.

AU - Ip, David C.W.

AU - Yow, Christine M.N.

N1 - Funding Information: We thank Biolitec for generously providing the photosensitizer FosPegr. This study was supported by The Hong Kong Polytechnic University Central Research Grant (G-YJ67 and G-U737).

PY - 2015/9

Y1 - 2015/9

N2 - Photodynamic therapy (PDT) is a novel therapeutic approach for combating various cancers. PDT involves the administration of a photosensitizer which generates singlet oxygen after light activation. FosPeg ® is the liposomal formulation of mTHPC. In this in vitro study, the photodynamic efficacy of FosPeg ® on a human colon cancer cell line (HT29) was investigated via studying the cellular uptake of FosPeg ®, FosPeg ® PDT mediated photocytotoxicity and the cell death mechanism were triggered. FosPeg ® PDT demonstrated its antitumor effect in a drug and light dose-dependent manner in HT-29 cells. Lethal dose (LD50) was achieved with 0.4 μg/mL of drug and 3 J/cm-2 of light dose. FosPeg ® PDT triggered apoptotic cell death via activating caspase cascade and regulating cell cycle progression. In conclusion, FosPeg ®-PDT is an effective measure to combat human colon cancer cells.

AB - Photodynamic therapy (PDT) is a novel therapeutic approach for combating various cancers. PDT involves the administration of a photosensitizer which generates singlet oxygen after light activation. FosPeg ® is the liposomal formulation of mTHPC. In this in vitro study, the photodynamic efficacy of FosPeg ® on a human colon cancer cell line (HT29) was investigated via studying the cellular uptake of FosPeg ®, FosPeg ® PDT mediated photocytotoxicity and the cell death mechanism were triggered. FosPeg ® PDT demonstrated its antitumor effect in a drug and light dose-dependent manner in HT-29 cells. Lethal dose (LD50) was achieved with 0.4 μg/mL of drug and 3 J/cm-2 of light dose. FosPeg ® PDT triggered apoptotic cell death via activating caspase cascade and regulating cell cycle progression. In conclusion, FosPeg ®-PDT is an effective measure to combat human colon cancer cells.

KW - apoptosis

KW - caspase-3

KW - FosPeg ®

KW - HT-29

U2 - 10.1142/S1793545815500273

DO - 10.1142/S1793545815500273

M3 - Article

AN - SCOPUS:84940034405

SN - 1793-5458

VL - 8

JO - Journal of Innovative Optical Health Sciences

JF - Journal of Innovative Optical Health Sciences

IS - 5

M1 - 1550027

ER -

An in vitro investigation of photodynamic efficacy of FosPeg® on human colon cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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