An expanded and flexible form of the vacuolar ATPase membrane sector

Daniel K. Clare, Elena V. Orlova, Malcolm E. Finbow, Michael A. Harrison, John B.C. Findlay, Helen R. Saibil

Research output: Contribution to journalArticle

Abstract

The vacuolar ATPase integral membrane c-ring from Nephrops norvegicus occurs in paired complexes in a double membrane. Using cryo-electron microscopy and single particle image processing of 2D crystals, we have obtained a projection structure of the c-ring of N. norvegicus. The c-ring was found to be very flexible, most likely as a result of an expanded conformation of the c subunits. This structure may support a role for the vacuolar ATPase c-rings in membrane fusion.

Original languageEnglish
JournalStructure
DOIs
Publication statusPublished - 1 Jul 2006

Fingerprint

Vacuolar Proton-Translocating ATPases
Membranes
Electron microscopy
Conformations
Image processing
Fusion reactions
Crystals

Keywords

  • cryo-electron microscopy
  • vacuolar ATPase

Cite this

Clare, D. K., Orlova, E. V., Finbow, M. E., Harrison, M. A., Findlay, J. B. C., & Saibil, H. R. (2006). An expanded and flexible form of the vacuolar ATPase membrane sector. Structure. https://doi.org/10.1016/j.str.2006.05.014
Clare, Daniel K. ; Orlova, Elena V. ; Finbow, Malcolm E. ; Harrison, Michael A. ; Findlay, John B.C. ; Saibil, Helen R. / An expanded and flexible form of the vacuolar ATPase membrane sector. In: Structure. 2006.
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Clare, DK, Orlova, EV, Finbow, ME, Harrison, MA, Findlay, JBC & Saibil, HR 2006, 'An expanded and flexible form of the vacuolar ATPase membrane sector', Structure. https://doi.org/10.1016/j.str.2006.05.014

An expanded and flexible form of the vacuolar ATPase membrane sector. / Clare, Daniel K.; Orlova, Elena V.; Finbow, Malcolm E.; Harrison, Michael A.; Findlay, John B.C.; Saibil, Helen R.

In: Structure, 01.07.2006.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An expanded and flexible form of the vacuolar ATPase membrane sector

AU - Clare, Daniel K.

AU - Orlova, Elena V.

AU - Finbow, Malcolm E.

AU - Harrison, Michael A.

AU - Findlay, John B.C.

AU - Saibil, Helen R.

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PY - 2006/7/1

Y1 - 2006/7/1

N2 - The vacuolar ATPase integral membrane c-ring from Nephrops norvegicus occurs in paired complexes in a double membrane. Using cryo-electron microscopy and single particle image processing of 2D crystals, we have obtained a projection structure of the c-ring of N. norvegicus. The c-ring was found to be very flexible, most likely as a result of an expanded conformation of the c subunits. This structure may support a role for the vacuolar ATPase c-rings in membrane fusion.

AB - The vacuolar ATPase integral membrane c-ring from Nephrops norvegicus occurs in paired complexes in a double membrane. Using cryo-electron microscopy and single particle image processing of 2D crystals, we have obtained a projection structure of the c-ring of N. norvegicus. The c-ring was found to be very flexible, most likely as a result of an expanded conformation of the c subunits. This structure may support a role for the vacuolar ATPase c-rings in membrane fusion.

KW - cryo-electron microscopy

KW - vacuolar ATPase

U2 - 10.1016/j.str.2006.05.014

DO - 10.1016/j.str.2006.05.014

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Clare DK, Orlova EV, Finbow ME, Harrison MA, Findlay JBC, Saibil HR. An expanded and flexible form of the vacuolar ATPase membrane sector. Structure. 2006 Jul 1. https://doi.org/10.1016/j.str.2006.05.014