Amoxicillin removal by pre-denitrification membrane bioreactor (A/OMBR): performance evaluation, degradation by-products, and antibiotic resistant bacteria

Milena Emy Matsubara; Karin Helwig; Colin Hunter; Joanne Roberts; Eduardo Lucas Subtil; Lucia Helena Gomes Coelho

doi:10.1016/j.ecoenv.2020.110258

Amoxicillin removal by pre-denitrification membrane bioreactor (A/OMBR): performance evaluation, degradation by-products, and antibiotic resistant bacteria

Milena Emy Matsubara, Karin Helwig, Colin Hunter, Joanne Roberts, Eduardo Lucas Subtil, Lucia Helena Gomes Coelho^*

^*Corresponding author for this work

Research output: Contribution to journal › Article

Abstract

Membrane bioreactors (MBRs) are one of the treatment technologies with the potential to remove emergingcompounds from wastewater. The present work evaluated the efficiency of an MBR pilot system in removingamoxicillin from synthetic wastewater using a continuous flow pre-denitrification MBR (A/O-MBR) pilot unit.The system operated in three phases: (1) synthetic wastewater and hydraulic retention time (HRT) of 40 h; (2)adding amoxicillin 100 μg L−1 to the influent, and (3) varying flowrate to HRT of 20 h. Liquid chromatographycoupled to high resolution mass spectrometry analysis confirmed the presence of five amoxicillin degradationby-products in the effluent. The addition of amoxicillin did not affect chemical oxygen demand (COD) or dissolvedorganic carbon (DOC) removal efficiencies. Respirometry showed that amoxicillin level did not inhibitheterotrophic bacteria metabolism. The change in HRT reduced the DOC removal (from 84% to 66%) but did notinfluence COD (> 94%) or total nitrogen (> 72%). The amoxicillin and by-products removal decreased from80% to 54% with HRT change. Adsorption and biodegradation represented the largest removed fraction of theantibiotic in the A/O-MBR system (68%). Ecotoxicity assays showed P. fluorescens was more resistant and E. coliless resistant to amoxicillin residues at effluent sample matrix.

Original language	English
Article number	110258
Journal	Ecotoxicology and Environmental Safety
Volume	192
Early online date	6 Feb 2020
DOIs	https://doi.org/10.1016/j.ecoenv.2020.110258
Publication status	Published - 1 Apr 2020

Keywords

bacteria resistance
degradation by-products
emerging contaminants
micropollutants
removal mechanisms

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10.1016/j.ecoenv.2020.110258

Embargoed Document

Matsubara, M.E. et al (2020) Amoxicillin removal by pre-denitrification membrane bioreactor (A/OMBR): Performance evaluation, degradation by-products, and antibiotic resistant bacteria
Accepted author manuscript, 1.34 MB
Licence: CC BY-NC-ND
Embargo ends: 6/02/21

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Matsubara, M. E., Helwig, K., Hunter, C., Roberts, J., Subtil, E. L., & Coelho, L. H. G. (2020). Amoxicillin removal by pre-denitrification membrane bioreactor (A/OMBR): performance evaluation, degradation by-products, and antibiotic resistant bacteria. Ecotoxicology and Environmental Safety, 192, [110258]. https://doi.org/10.1016/j.ecoenv.2020.110258

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abstract = "Membrane bioreactors (MBRs) are one of the treatment technologies with the potential to remove emergingcompounds from wastewater. The present work evaluated the efficiency of an MBR pilot system in removingamoxicillin from synthetic wastewater using a continuous flow pre-denitrification MBR (A/O-MBR) pilot unit.The system operated in three phases: (1) synthetic wastewater and hydraulic retention time (HRT) of 40 h; (2)adding amoxicillin 100 μg L−1 to the influent, and (3) varying flowrate to HRT of 20 h. Liquid chromatographycoupled to high resolution mass spectrometry analysis confirmed the presence of five amoxicillin degradationby-products in the effluent. The addition of amoxicillin did not affect chemical oxygen demand (COD) or dissolvedorganic carbon (DOC) removal efficiencies. Respirometry showed that amoxicillin level did not inhibitheterotrophic bacteria metabolism. The change in HRT reduced the DOC removal (from 84% to 66%) but did notinfluence COD (> 94%) or total nitrogen (> 72%). The amoxicillin and by-products removal decreased from80% to 54% with HRT change. Adsorption and biodegradation represented the largest removed fraction of theantibiotic in the A/O-MBR system (68%). Ecotoxicity assays showed P. fluorescens was more resistant and E. coliless resistant to amoxicillin residues at effluent sample matrix.",

keywords = "bacteria resistance, degradation by-products, emerging contaminants, micropollutants, removal mechanisms",

author = "Matsubara, {Milena Emy} and Karin Helwig and Colin Hunter and Joanne Roberts and Subtil, {Eduardo Lucas} and Coelho, {Lucia Helena Gomes}",

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Amoxicillin removal by pre-denitrification membrane bioreactor (A/OMBR): performance evaluation, degradation by-products, and antibiotic resistant bacteria. / Matsubara, Milena Emy; Helwig, Karin ; Hunter, Colin ; Roberts, Joanne; Subtil, Eduardo Lucas; Coelho, Lucia Helena Gomes.

In: Ecotoxicology and Environmental Safety, Vol. 192, 110258, 01.04.2020.

Research output: Contribution to journal › Article

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AU - Matsubara, Milena Emy

AU - Helwig, Karin

AU - Hunter, Colin

AU - Roberts, Joanne

AU - Subtil, Eduardo Lucas

AU - Coelho, Lucia Helena Gomes

N1 - Acceptance from webpage AAM: 12m embargo

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AB - Membrane bioreactors (MBRs) are one of the treatment technologies with the potential to remove emergingcompounds from wastewater. The present work evaluated the efficiency of an MBR pilot system in removingamoxicillin from synthetic wastewater using a continuous flow pre-denitrification MBR (A/O-MBR) pilot unit.The system operated in three phases: (1) synthetic wastewater and hydraulic retention time (HRT) of 40 h; (2)adding amoxicillin 100 μg L−1 to the influent, and (3) varying flowrate to HRT of 20 h. Liquid chromatographycoupled to high resolution mass spectrometry analysis confirmed the presence of five amoxicillin degradationby-products in the effluent. The addition of amoxicillin did not affect chemical oxygen demand (COD) or dissolvedorganic carbon (DOC) removal efficiencies. Respirometry showed that amoxicillin level did not inhibitheterotrophic bacteria metabolism. The change in HRT reduced the DOC removal (from 84% to 66%) but did notinfluence COD (> 94%) or total nitrogen (> 72%). The amoxicillin and by-products removal decreased from80% to 54% with HRT change. Adsorption and biodegradation represented the largest removed fraction of theantibiotic in the A/O-MBR system (68%). Ecotoxicity assays showed P. fluorescens was more resistant and E. coliless resistant to amoxicillin residues at effluent sample matrix.

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KW - emerging contaminants

KW - micropollutants

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