Airway epithelial inflammatory responses and clinical parameters in COPD

I. S. Patel, N.J. Roberts, S.J. Lloyd-Owen, R. J. Sapsford, J.A. Wedzicha

    Research output: Contribution to journalArticle

    Abstract

    This study examined inflammatory responses from primary cultured human bronchial epithelial cells in chronic obstructive pulmonary disease (COPD) and the clinical factors modulating them. Epithelial cells from bronchoscopic biopsies from 14 patients with COPD ((mean¡SD) age 74.6¡5.7 yrs, forced expiratory volume in one second (FEV1) 1.21¡0.36 L, FEV1 % predicted 51.1¡15.8%, 51.5¡24.0 pack-yrs of smoking, inhaled steroid dosage 1237.5¡671.0 mg?day-1, Medical Research Council (MRC) dyspnoea score 3.18¡1.33) and eight current/exsmokers with normal pulmonary function (age 60.4¡13.5 yrs, FEV1 2.66¡1.27 L, FEV1 % pred 89.6¡17.7%, 49¡44 pack-yrs of smoking, MRC dyspnoea score 1¡0) were grown in primary culture and exposed to 50 ng?mL-1 tumour necrosis factor-a. Stimulated COPD cells produced significantly more interleukin (IL)-6 at 24 and 48 h, and IL-8 at 6 and 24 h than unstimulated COPD cells. This response was not seen in cells from current/exsmokers. IL-6 and IL-8 production was lower in COPD patients taking inhaled steroids. Following an inflammatory stimulus, bronchial epithelial cells in chronic obstructive pulmonary disease show a significant cytokine response not seen in smokers with normal pulmonary function and this may be modified by inhaled steroid therapy.
    Original languageEnglish
    Pages (from-to)94-99
    Number of pages5
    JournalEuropean Respiratory Journal
    Volume22
    DOIs
    Publication statusPublished - 2003

    Keywords

    • airway chronic obstructive pulmonary disease
    • corticosteroids
    • epithelium
    • inflammation

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