Abstract
Three-dimensional (3D) organotypic models are increasingly being used to study aspects of epidermal organisation and cutaneous wound-healing events. These are largely dependent on laborious histological analysis and immunohistochemical approaches. Here we outline a method for establishment of a versatile in vitro 3D organotypic skin equivalent that reflects murine epidermal organisation in vivo. The system is optically transparent and ideally suited to real-time analysis and integrated in situ imaging techniques.
Original language | English |
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Title of host publication | Epidermal Cells: Methods and Protocols |
Publisher | Springer Nature |
Pages | 303-312 |
Number of pages | 10 |
ISBN (Print) | 9781607613794 |
Publication status | Published - 1 Jan 2009 |
Keywords
- connexin
- wound healing
- epidermal organisation