Three-dimensional (3D) organotypic models are increasingly being used to study aspects of epidermal organisation and cutaneous wound-healing events. These are largely dependent on laborious histological analysis and immunohistochemical approaches. Here we outline a method for establishment of a versatile in vitro 3D organotypic skin equivalent that reflects murine epidermal organisation in vivo. The system is optically transparent and ideally suited to real-time analysis and integrated in situ imaging techniques.
|Title of host publication||Epidermal Cells: Methods and Protocols|
|Number of pages||10|
|Publication status||Published - 1 Jan 2009|
- wound healing
- epidermal organisation