A versatile murine 3D organotypic model to evaluate aspects of wound healing and epidermal organisation

Eve Kandyba, Malcolm B. Hogins, Patricia E.M. Martin

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Three-dimensional (3D) organotypic models are increasingly being used to study aspects of epidermal organisation and cutaneous wound-healing events. These are largely dependent on laborious histological analysis and immunohistochemical approaches. Here we outline a method for establishment of a versatile in vitro 3D organotypic skin equivalent that reflects murine epidermal organisation in vivo. The system is optically transparent and ideally suited to real-time analysis and integrated in situ imaging techniques.

Original languageEnglish
Title of host publicationEpidermal Cells: Methods and Protocols
PublisherSpringer
Pages303-312
Number of pages10
ISBN (Print)9781607613794
Publication statusPublished - 1 Jan 2009

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Keywords

  • connexin
  • wound healing
  • epidermal organisation

Cite this

Kandyba, E., Hogins, M. B., & Martin, P. E. M. (2009). A versatile murine 3D organotypic model to evaluate aspects of wound healing and epidermal organisation. In Epidermal Cells: Methods and Protocols (pp. 303-312). Springer.