A systematic approach for assessing Ca²⁺ handling in cardiac myocytes

Karin R. Sipido; Niall Macquaide; Virginie Bito

doi:10.1101/pdb.top066142

A systematic approach for assessing Ca²⁺ handling in cardiac myocytes

Karin R. Sipido^*, Niall Macquaide, Virginie Bito

^*Corresponding author for this work

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

In cardiac myocytes, Ca(2+) release from the sarcoplasmic reticulum (SR) Ca(2+) store through the opening of ryanodine receptors (RyRs) is the major source of Ca(2+) for activation of myofilaments and contraction. Over the past 20 years, tools have become available to study this release process in detail, allowing new insights into the regulation of SR Ca(2+) release and RyR function. To assess these processes, we recommend and here review a systematic approach that evaluates the essential transport mechanisms and Ca(2+) fluxes in isolated single cardiac myocytes by using fluorescent Ca(2+) indicators and whole-cell recording of membrane voltage and ionic currents under voltage clamp. The approach includes an assessment of the L-type Ca(2+) current as a trigger for opening of RyRs and release of SR Ca(2+), of the SR Ca(2+) content, of intrinsic properties of RyRs, and of Ca(2+)-removal systems.

Original language	English
Pages (from-to)	431-3
Number of pages	3
Journal	Cold Spring Harbor protocols
Volume	2015
Issue number	5
DOIs	https://doi.org/10.1101/pdb.top066142
Publication status	Published - 1 May 2015

Keywords

Calcium/metabolism
Cytological Techniques/methods
Muscle Contraction
Myocytes, Cardiac/metabolism
Myofibrils/metabolism
Optical Imaging/methods
Patch-Clamp Techniques/methods
Ryanodine Receptor Calcium Release Channel/metabolism
Staining and Labeling/methods

Documents and Links

10.1101/pdb.top066142

Fingerprint Dive into the research topics of 'A systematic approach for assessing Ca²⁺ handling in cardiac myocytes'. Together they form a unique fingerprint.

Cite this

@article{b732bbb6675843dea6c19653c96c4969,

title = "A systematic approach for assessing Ca²⁺ handling in cardiac myocytes",

abstract = "In cardiac myocytes, Ca(2+) release from the sarcoplasmic reticulum (SR) Ca(2+) store through the opening of ryanodine receptors (RyRs) is the major source of Ca(2+) for activation of myofilaments and contraction. Over the past 20 years, tools have become available to study this release process in detail, allowing new insights into the regulation of SR Ca(2+) release and RyR function. To assess these processes, we recommend and here review a systematic approach that evaluates the essential transport mechanisms and Ca(2+) fluxes in isolated single cardiac myocytes by using fluorescent Ca(2+) indicators and whole-cell recording of membrane voltage and ionic currents under voltage clamp. The approach includes an assessment of the L-type Ca(2+) current as a trigger for opening of RyRs and release of SR Ca(2+), of the SR Ca(2+) content, of intrinsic properties of RyRs, and of Ca(2+)-removal systems. ",

keywords = "Calcium/metabolism, Cytological Techniques/methods, Muscle Contraction, Myocytes, Cardiac/metabolism, Myofibrils/metabolism, Optical Imaging/methods, Patch-Clamp Techniques/methods, Ryanodine Receptor Calcium Release Channel/metabolism, Staining and Labeling/methods",

author = "Sipido, {Karin R.} and Niall Macquaide and Virginie Bito",

note = "No acceptance date - ELL 11/6/20 {\textcopyright} 2015 Cold Spring Harbor Laboratory Press.",

year = "2015",

month = may,

day = "1",

doi = "10.1101/pdb.top066142",

language = "English",

volume = "2015",

pages = "431--3",

journal = "Cold Spring Harbor protocols",

issn = "1559-6095",

publisher = "Cold Spring Harbor Laboratory Press",

number = "5",

}

TY - JOUR

T1 - A systematic approach for assessing Ca²⁺ handling in cardiac myocytes

AU - Sipido, Karin R.

AU - Macquaide, Niall

AU - Bito, Virginie

PY - 2015/5/1

Y1 - 2015/5/1

N2 - In cardiac myocytes, Ca(2+) release from the sarcoplasmic reticulum (SR) Ca(2+) store through the opening of ryanodine receptors (RyRs) is the major source of Ca(2+) for activation of myofilaments and contraction. Over the past 20 years, tools have become available to study this release process in detail, allowing new insights into the regulation of SR Ca(2+) release and RyR function. To assess these processes, we recommend and here review a systematic approach that evaluates the essential transport mechanisms and Ca(2+) fluxes in isolated single cardiac myocytes by using fluorescent Ca(2+) indicators and whole-cell recording of membrane voltage and ionic currents under voltage clamp. The approach includes an assessment of the L-type Ca(2+) current as a trigger for opening of RyRs and release of SR Ca(2+), of the SR Ca(2+) content, of intrinsic properties of RyRs, and of Ca(2+)-removal systems.

AB - In cardiac myocytes, Ca(2+) release from the sarcoplasmic reticulum (SR) Ca(2+) store through the opening of ryanodine receptors (RyRs) is the major source of Ca(2+) for activation of myofilaments and contraction. Over the past 20 years, tools have become available to study this release process in detail, allowing new insights into the regulation of SR Ca(2+) release and RyR function. To assess these processes, we recommend and here review a systematic approach that evaluates the essential transport mechanisms and Ca(2+) fluxes in isolated single cardiac myocytes by using fluorescent Ca(2+) indicators and whole-cell recording of membrane voltage and ionic currents under voltage clamp. The approach includes an assessment of the L-type Ca(2+) current as a trigger for opening of RyRs and release of SR Ca(2+), of the SR Ca(2+) content, of intrinsic properties of RyRs, and of Ca(2+)-removal systems.

KW - Calcium/metabolism

KW - Cytological Techniques/methods

KW - Muscle Contraction

KW - Myocytes, Cardiac/metabolism

KW - Myofibrils/metabolism

KW - Optical Imaging/methods

KW - Patch-Clamp Techniques/methods

KW - Ryanodine Receptor Calcium Release Channel/metabolism

KW - Staining and Labeling/methods

U2 - 10.1101/pdb.top066142

DO - 10.1101/pdb.top066142

M3 - Article

C2 - 25934941

VL - 2015

SP - 431

EP - 433

JO - Cold Spring Harbor protocols

JF - Cold Spring Harbor protocols

SN - 1559-6095

IS - 5

ER -